WO2000047755A3 - Plant udp-glucose epimerases - Google Patents

Plant udp-glucose epimerases Download PDF

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Publication number
WO2000047755A3
WO2000047755A3 PCT/US2000/003453 US0003453W WO0047755A3 WO 2000047755 A3 WO2000047755 A3 WO 2000047755A3 US 0003453 W US0003453 W US 0003453W WO 0047755 A3 WO0047755 A3 WO 0047755A3
Authority
WO
WIPO (PCT)
Prior art keywords
udp
epimerases
glucose
epimerase
galactose
Prior art date
Application number
PCT/US2000/003453
Other languages
French (fr)
Other versions
WO2000047755A2 (en
Inventor
Thomas J Carlson
Gary M Fader
Omolayo O Famodu
Anthony J Kinney
Richard W Pearlstein
J Antoni Rafalski
Catherine J Thorpe
Original Assignee
Du Pont
Thomas J Carlson
Gary M Fader
Omolayo O Famodu
Anthony J Kinney
Richard W Pearlstein
J Antoni Rafalski
Catherine J Thorpe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Thomas J Carlson, Gary M Fader, Omolayo O Famodu, Anthony J Kinney, Richard W Pearlstein, J Antoni Rafalski, Catherine J Thorpe filed Critical Du Pont
Priority to AU32273/00A priority Critical patent/AU3227300A/en
Priority to US09/913,064 priority patent/US6992236B1/en
Priority to EP00910131A priority patent/EP1151115A2/en
Priority to CA2359803A priority patent/CA2359803C/en
Publication of WO2000047755A2 publication Critical patent/WO2000047755A2/en
Publication of WO2000047755A3 publication Critical patent/WO2000047755A3/en
Priority to US11/106,270 priority patent/US7294762B2/en
Priority to US11/924,008 priority patent/US7741535B2/en
Priority to US12/787,453 priority patent/US7968766B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

Abstract

This invention relates to an isolated nucleic acid fragment encoding a UDP-galactose 4-epimerase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the UDP-galactose 4-epimerase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the UDP-galactose 4-epimerase in a transformed host cell.
PCT/US2000/003453 1999-02-10 2000-02-09 Plant udp-glucose epimerases WO2000047755A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU32273/00A AU3227300A (en) 1999-02-10 2000-02-09 Udp-glucose modifiers
US09/913,064 US6992236B1 (en) 1999-02-10 2000-02-09 Plant UDP-glucose epimerases
EP00910131A EP1151115A2 (en) 1999-02-10 2000-02-09 Plant udp-glucose epimerases
CA2359803A CA2359803C (en) 1999-02-10 2000-02-09 Udp-glucose epimerases
US11/106,270 US7294762B2 (en) 1999-02-10 2005-04-14 Plant UDP-galactose epimerases
US11/924,008 US7741535B2 (en) 1999-02-10 2007-10-25 Plant UDP-galactose epimerases
US12/787,453 US7968766B2 (en) 1999-02-10 2010-05-26 Plant UDP-galatose epimerases

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11958899P 1999-02-10 1999-02-10
US60/119,588 1999-02-10

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09913064 A-371-Of-International 2000-02-09
US11/106,270 Division US7294762B2 (en) 1999-02-10 2005-04-14 Plant UDP-galactose epimerases

Publications (2)

Publication Number Publication Date
WO2000047755A2 WO2000047755A2 (en) 2000-08-17
WO2000047755A3 true WO2000047755A3 (en) 2001-03-08

Family

ID=22385197

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2000/003513 WO2000047748A2 (en) 1999-02-10 2000-02-09 Udp-glucose modifiers
PCT/US2000/003453 WO2000047755A2 (en) 1999-02-10 2000-02-09 Plant udp-glucose epimerases

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2000/003513 WO2000047748A2 (en) 1999-02-10 2000-02-09 Udp-glucose modifiers

Country Status (5)

Country Link
EP (2) EP1151111A2 (en)
AR (1) AR022567A1 (en)
AU (2) AU3227300A (en)
CA (3) CA2716429A1 (en)
WO (2) WO2000047748A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030073828A1 (en) * 2001-02-01 2003-04-17 Battelle Memorial Institute Epimerase gene and use thereof
CN103725658B (en) * 2013-12-23 2015-11-18 内蒙古大学 Derive from the plant development associated protein LgUGPase of larch in Xinanlin area and encoding gene thereof and application

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998054335A1 (en) * 1997-05-28 1998-12-03 Danisco A/S In vivo modification of galactomannans in guar by expression of udp-galactose epimerase antisense rna

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5646023A (en) * 1993-04-15 1997-07-08 J.R. Simplot Company Modulation of sugar content in plants
JPH07327679A (en) * 1994-06-06 1995-12-19 Toyota Motor Corp Dna sequence coding udp glucose synthetase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998054335A1 (en) * 1997-05-28 1998-12-03 Danisco A/S In vivo modification of galactomannans in guar by expression of udp-galactose epimerase antisense rna

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DÖRMANN ET AL: "Functional expression of Uridine 5'diphospho-glucose 4-epimerase (EC 5.1.3.2) from Arabidopsis thaliana in Saccharomyces cerevisiae and Escherichia coli", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,US,NEW YORK, US, vol. 327, 1996, pages 27 - 34, XP002076759, ISSN: 0003-9861 *
DÖRMANN ET AL: "The role of UDP-glucose epimerase in carbohydrate metabolism of Arabidopsis", PLANT JOURNAL,GB,BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, vol. 13, no. 5, 1 March 1998 (1998-03-01), pages 641 - 652, XP002082772, ISSN: 0960-7412 *
LAKE MARC R ET AL: "Molecular cloning and characterization of a UDP-glucose-4-epimerase gene (galE) and its expression in pea tissues.", PLANT PHYSIOLOGY AND BIOCHEMISTRY (PARIS), vol. 36, no. 8, August 1998 (1998-08-01), pages 555 - 562, XP000923481, ISSN: 0981-9428 *
WALBOT V.: "Maize EST AC AW267428", EBI DATABASE, 6 January 2000 (2000-01-06), XP002142269 *

Also Published As

Publication number Publication date
WO2000047748A2 (en) 2000-08-17
CA2359811A1 (en) 2000-08-17
AU3228300A (en) 2000-08-29
CA2359803C (en) 2013-04-09
EP1151111A2 (en) 2001-11-07
CA2716429A1 (en) 2000-08-17
AR022567A1 (en) 2002-09-04
EP1151115A2 (en) 2001-11-07
AU3227300A (en) 2000-08-29
CA2359811C (en) 2010-12-21
WO2000047755A2 (en) 2000-08-17
WO2000047748A3 (en) 2000-12-14
CA2359803A1 (en) 2000-08-17

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