WO1999012950A2 - Improvements in or relating to stability of plant starches - Google Patents
Improvements in or relating to stability of plant starches Download PDFInfo
- Publication number
- WO1999012950A2 WO1999012950A2 PCT/GB1998/002665 GB9802665W WO9912950A2 WO 1999012950 A2 WO1999012950 A2 WO 1999012950A2 GB 9802665 W GB9802665 W GB 9802665W WO 9912950 A2 WO9912950 A2 WO 9912950A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- plant
- starch
- sequence
- nucleic acid
- isoamylase
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
- C12N9/2457—Pullulanase (3.2.1.41)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
- C12N9/246—Isoamylase (3.2.1.68)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01041—Pullulanase (3.2.1.41)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01068—Isoamylase (3.2.1.68)
Definitions
- This invention relates to novel nucleotide sequences, nucleic acid constructs and host cells and organisms comprising said sequences, methods of altering plants using the sequences, and to products obtainable from the altered plants.
- Starch the major storage reserve in plants, consists of two main polysaccharides, amylose and amylopectin.
- Amylose is a linear polymer containing ⁇ -1,4 linked glucose units
- amylopectin is a highly branched polymer consisting of a ⁇ -1,4 linked glucan backbone with ⁇ -1,6 linked glucan branches.
- amylopectin constitutes about 75% of the starch content.
- starch synthases which elongate the glucan chains
- SBE starch branching enzymes
- Debranching enzymes hydrolyse the ⁇ l-6-glucosidic linkages of ⁇ -glucan polymers and are classified into two types depending on their substrate specificities: (1) pullulanases (EC 3.2.1.41 , which are known in plants as "R- enzymes") which act on amylopectin and puUulan (a linear bacterial polymer consisting of 1-6 linked maltotriose units) but which cannot use glycogen as a substrate; and (2) isoamylases (EC 3.2.1.68) which act on amylopectin and glycogen but cannot use puUulan as a substrate.
- pullulanases EC 3.2.1.41 , which are known in plants as "R- enzymes”
- puUulan a linear bacterial polymer consisting of 1-6 linked maltotriose units
- Pullulanases have been purified from a number of plants including maize, rice, sorghum, oat, broad bean, potato, sugarbeet and spinach and the corresponding cDNA clones of maize, rice and spinach have been isolated (James et al 1995; Nakamura et al 1996; Renz et al 1995). In potato, both R-enzyme and isoamylase enzyme polypeptides have been characterised and purified (Drummond et al 1970; Ishizaki,Y et al 1983). A recent publication (WO 95/04826) detailed the purification and peptide sequence determination of a pullulanase type DBE.
- a cDNA encoding a potato DBE was described in a second patent application (WO 96/19581) which detailed the isolation of a partial length (492 bp) cDNA clone encoding a pullulanase type DBE, as well as the effect of downregulating the expression of this gene in transgenic antisense plants.
- An unspecified number of lines were generated and some showed a decrease in debranching enzyme activity (not quantitated) which also led to a slight modification in the swelling characteristics of the starch as measured by RVA (Rapid Viscoamylograph).
- the present application relates particularly to the isolation of nucleic acid sequences encoding potato debranching enzymes (especially isoamylase type debranching enzymes) and to the uses thereof.
- the invention provides a nucleic acid sequence obtainable from potato plants and carrying at least a portion of an isoamylase-type debranching enzyme (DBE) gene.
- DBE isoamylase-type debranching enzyme
- the nucleic acid sequence will comprise at least one region which is characteristic of isoamylase-type debranching enzymes and which is not present in pullulanase type debranching enzymes.
- the nucleic acid sequence will preferably be at least 200bp long, more preferably at least 300-600bp long. Conveniently the sequence is one which is derived or obtained from potato plants.
- the nucleic acid sequence of the invention may be, for instance, a genomic sequence, or may be a cDNA sequence.
- the nucleic acid sequence of the invention may comprise non- coding portions (which are transcribed, but not translated), and/or may comprise coding portions, which are translated into amino acid residues in the full length native gene. As described below, either translated or non-translated portions may be useful in certain techniques, and both therefore possess utility.
- the nucleic acid sequence comprises nucleotides 79-1595 of the nucleic acid sequence shown in Figure 8, or a functional equivalent thereof.
- This sequence includes a portion encoding the amino acid sequence MDWF/YNH, which portion is typically present in the nucleic acid sequences of the invention.
- Functionally equivalent nucleic acid sequences include sequences which encode substantially the same polypeptide, but which differ in nucleotide sequence from mat shown in Figure 8 by virtue of the degeneracy of the genetic code.
- a nucleic acid sequence may be altered (e.g.
- nucleotide sequence differs substantially whilst the amino acid sequence of the encoded polypeptide is unchanged.
- functionally equivalent nucleic acid sequences are those- which will hybridise under stringent hybridisation -conditions (e.g..as- described by Sambrook et al, Molecular Cloning. A Laboratory Manual, CSH, i.e. washing with O. lxSSC, 0.5% SDS at 68°C) with the sequence shown in Figure 8.
- Figures_6A-7B illustrate some related sequences, which are also in accordance with the present invention. These are genomic sequences with large stretches of intron sequence, which introns are not represented in rnRNA. The sequences illustrated in Figures 6A-7B thus comprise both coding and non-coding portions.
- DNA sequences functionally equivalent to that shown in Figure 8 will preferably comprise at least 200, more preferably 300-600bp, and typically will exhibit at least 85% identity (preferably at least 90%, and more preferably at least 95% identity) with the corresponding region of the DNA sequence shown in Figure 8.
- Those skilled in the an will readily be able to conduct a sequence alignment between the putative functionally equivalent sequence and that detailed in Figure 8 - the identity of the two sequences is to be compared in those regions which are aligned by standard computer software, which aligns corresponding regions of the sequences.
- the invention provides a replicable nucleic acid construct comprising the nucleic acid sequence of the first aspect defined above.
- the construct may typically comprise a selectable marker (e.g. kanamycin or hygromycin-resistance) and may allow for transcription (and possibly translation) of the nucleic acid sequence of the invention.
- a selectable marker e.g. kanamycin or hygromycin-resistance
- the construct will comprise a promoter (especially a promoter sequence operable in a plant and/or a promoter operable in a bacterial cell), and one or more regulatory signals (such as polyadenylation signals) known to those skilled in the art.
- the nucleic acid sequence of the invention can be introduced into plants which express a homologous sequence encoding an isoamylase enzyme, in such a way that the introduced sequence interferes with the expression of the homologous sequence.
- the nucleic acid sequence of the invention may be operably linked in the antisense orientation to a suitable promoter active in the plant into which the sequence is to be introduced, so as to reduce the levels of expression of the homologous isoamylase enzyme present in the plant-. _ -
- sense suppression can also occur (i.e. expression of an introduced sequence operably linked in the sense orientation can interfere, by some unknown mechanism, with the expression of the homologous, native gene), as described by Matzke & Matzke (1995 Plant Physiol. 107, 679-685). Any one of the methods mentioned by Matzke & Matzke could, in theory, be used to affect the expression in a host of a homologous isoamylase gene.
- antisense methods are mainly operable by the production of antisense mRNA which hybridises to the sense mRNA, preventing its translation into functional polypeptide, possibly by causing the hybrid RNA to be degraded (e.g. Sheehy et al, 1988 PNAS 55, 8805-8809; Van der Krol et al, Mol. Gen. Genet. 220, 204-212).
- Sense suppression also requires homology between the introduced sequence and the target gene, but the exact mechanism is unclear.
- nucleic acid used will comprise at least 300-600bp of sequence, but by simple trial and error other fragments (smaller or larger) may be found which are functional in altering the characteristics of the plant.
- the invention provides a method of altering a plant by inhibiting the expression of an isoamylase gene therein, the method comprising introducing into the plant the nucleic acid sequence of the invention, operably linked (in the sense or anti-sense orientation) to a promoter active in the plant, wherein the isoamylase gene in the plant has homology to the introduced sequence.
- the introduced nucleic acid will typically comprise at least 200bp, more preferably 300-600bp of sequence having at least 80% (advantageously at least 85%, desirably over 90%) sequence identity with the native isoamylase gene(s) present in the plant.
- the " plant will be a plant which normally comprises large amounts oi J starch, such as potato, sweet potato, maize, wheat, barley, oat, cassava, pea, rice and the like. It is probable that the greater the homology between the introduced sequence and the native gene, the greater the inhibition of expression of the native gene.
- the plant is therefore a potato plant, and/or a plant which has a reduced amylose content (e.g. the "waxy” mutation of potato or the "LAM” [low amylose mutant] strain of pea).
- Inhibition of the isoamylase gene might be expected to affect the properties of the starch present in the plant. In particular it might be expected to result in a starch having increased branching and/or shorter chain length. The obtaining of starch having such properties is a particular object of the present invention.
- nucleic acid sequences may also be advantageous to introduce into the plant one or more additional nucleic acid sequences, especially sequences which might also be expected to have an effect on synthesis and/or metabolism of starch in the plant.
- Particularly preferred are sequences which inhibit the expression of a pullulanase debranching enzyme in the plant.
- Plant pullulanase sequences are known to those skilled in the art (e.g. WO 96/19581), and a portion of a potato pullulanase cDNA sequence is also disclosed herein.
- the additional nucleic acid sequence will typically be homologous to a portion of a native gene in the plant and, as described above, may be introduced into the plant operably linked to a suitable promoter in either the sense or the anti-sense orientation.
- the introduced sequence of the invention and the one or more additional sequences may be operably linked to a single promoter (which would ensure both sequences were transcribed at essentially the same time), or may be operably linked to separate promoters (which may be necessary for optimal expression). Where separate promoters are employed they may be identical to each other or different. Suitable promoters are well known to those skilled in the art and include both constitutive and inducible types. Examples include the CaMV 35S promoter (e.g. single or tandem repeat) and the GBSS type I or patatin promoters of potato.
- the promoter will be tissue-specific. Desirably the promoter will cause expression of the operably linked sequence at substantial levels only in the tissue of the plant where starch synthesis and/or starch storage mainly occurs. Thus, for " example, where the sequence is introduced into a potato plant, the * operably linked promoter may be tuber-specific, such as the patatin promoter.
- nucleic acid sequences may be present on the same nucleic acid plant transformation vector as the portion of isoamylase-encoding sequence, or may be introduced on separate vectors (for example by re-transformation).
- sequence of the invention, and the one or more additional sequences if desired, can be introduced into the plant by any one of a number of well-known techniques (e.g. Agrobacterium-mediated transformation, or by "biolistic" methods).
- the invention provides a plant cell, or a plant or the progeny thereof, which has been altered by the method defined above.
- Altered plant cells, into which the sequence of the invention has been introduced may be grown into plantlets by conventional methods well-known to those skilled in the an.
- the progeny of an altered plant may be obtained, for example, by vegetative propagation, or by crossing the altered plant and reserving the seed so obtained.
- the invention also provides pans of the altered plant, such as storage organs.
- the invention provides tubers comprising altered starch, said tubers being obtained from an altered plant or the progeny thereof.
- the invention also provides a method of obtaining starch from an altered plant, the plant being obtained by the method defined above.
- Starch may be extracted from the plant by any of the known techniques (e.g. milling).
- the invention further provides starch obtainable from a plant altered by the method defined above, the starch having altered propenies compared to starch extracted from an equivalent but unaltered plant.
- the altered starch is obtained from an altered plant selected from the group consisting of cassava, potato, pea, tomato, maize, wheat, barley, oat, sweet potato and rice.
- the altered starch will have an increased degree of branching and/or reduced amylopectin chain length, compared to starch extracted from an equivalent but unaltered plant.
- such altered starch obtained by means of the present invention is found to possess different viscosity charcteristics compared to starch extracted from equivalent, but unaltered, plants.
- the inventors have found that starch obtained from plants altered by the method of the invention may have reduced peak viscosity compared to starch extracted from an equivalent but unaltered plant, as determined by analysis of a 7.4% (w/v) starch suspension using a Rapid Visco Analyser series 4 instrument, (available from Newport Scientific, Sydney, Australia) according to the manufacturer's standard stirring and heating protocol.
- the altered starch may have higher setback viscosity compared to starch extracted from an equivalent but unaltered plant, (as determined, for example, by analysis of a 7.4% (w/v) starch suspension using a Rapid Visco Analyser series 4 instrument). Further, the altered starch may have an increased viscosity onset temperature compared to starch extracted from an equivalent but unaltered plant, (as determined by analysis of a 7.4% (w/v) starch suspension using a Rapid Visco Analyser series 4 instrument).
- peak viscosity is well known and understood by the person skilled in the art.
- the viscosity of starch preparations is generally investigated by heating an aqueous sample of the starch under controlled conditions of temperature increase, temperature maintenance, and subsequent temperature decrease and with controlled stirring, using an instrument known as a Visco analyser, which produces a viscosity profile called a "viscoamylograph".
- the "peak viscosity” is the maximum viscosity which is attained during the temperature increase phase.
- setback viscosity is the viscosity obtained at the end of the viscoamylograph.
- the viscosity onset temperature is the temperature at which a sudden increase of viscosity is apparent on the viscoamylograph.
- an "equivalent, but unaltered” plant may be considered as a plant which has a substantially identical genotype to a plant altered by the method of the invention, except for the introduced sequences present in the altered plant of the invention. Also to be considered as an equivalent but unaltered plant are those plants which comprise the introduced sequence but wherein the introduced sequence has not affected the starch properties of the plant.
- the invention provides a nucleic acid sequence encoding at least a portion of a potato pullulanase type debranching enzyme, the sequence comprising- substantially nucleotides 46-774 of the nucleic acid sequence shown in Figure 10, or a functional equivalent thereof.
- the term "functional equivalent” should be interpreted as explained previously, but with reference (in this aspect of the invention) to Figure 10 rather than Figure 8.
- the invention provides nucleic acid constructs, and altered host plant cells and organisms, comprising the pullulanase-type DBE nucleic acid sequence defined immediately above.
- Figure 1 shows a comparison of the known amino acid sequences of various putative isoamylase proteins.
- the shaded area indicates residues (single letter amino acid code) that are identical to the maize protein. Dashes indicate gaps introduced to maximise the alignment. Although the whole sequences were compared, only the most conserved region is shown. Sequences are identified at the right; A10906_l is an artificial sequence, A37035 is from a Pseudomonas species SMP1, HIU32761 is from Haemophilus influenzae, ISOA_PSESP is from Pseudomonas amylodermosa and ZMU18908_1 is from Zea mays (corn);
- Figures 2A and 2B show a comparison of the 5' and 3' ends (respectively) of two partial pullulanase type DBE genomic clones recovered from potato by the present inventors (nucleotides that are identical are shown between the two sequences and dashes indicate gaps introduced to optimise the alignment);
- Figures 3A and 3B show the DNA sequence (SEQ. ID. NO.s 1 and 4 respectively) and predicted intron exon structure, including predicted amino acid sequences (SEQ. ID. NO.s 2 and 3) at the 5' and 3' ends respectively of a partial pullulanase type DBE genomic clone (certain restriction sites are also marked);
- Figures 4A and 4B show the DNA sequence (SEQ. ID. NO.s 5 and 8 respectively) and- predicted intron/exon structure, including predicted amino acid sequences (SEQ. ID. NO.s 6 and 7) at the 5' and 3' ends respectively of a partial pullulanase type DBE genomic clone (certain restriction sites are also marked);
- Figures 5 A and 5B show a comparison of the 5' and 3' ends (respectively) of two partial isoamylase type DBE genomic clones recovered from potato by the present inventors (nucleotides that are identical are shown between the two sequences and dashes indicate gaps introduced to optimise the alignment);
- Figures 6 A and 6B show the DNA sequence (SEQ. ID. NO.s 9 and 11 respectively) and predicted intron/exon strucmre, including predicted amino acid sequence (SEQ. ID. NO. 10) at the 5' and 3' ends respectively of a partial isoamylase type DBE genomic clone (certain restriction sites are also marked);
- Figures 7A and 7B show the DNA sequence (SEQ. ID. NO.s 12 and 13 respectively) and predicted intron/exon strucmre at the 5' and 3' ends respectively of a partial isoamylase type DBE genomic clone (certain restriction sites are also marked);
- Figure 8 shows the DNA sequence (SEQ. ID. NO. 14), restriction map and predicted open reading frame (including predicted amino acid sequence, SEQ. ID. NO. 15) of a partial length potato debranching enzyme (isoamylase) cDNA clone (pSJ132, described below); nucleotides 79-1595, including the poly(A) tail, are isoamylase, the other sequence is derived from pT7Blue vector;
- Figure 9 is a schematic representation of the plant transformation vector pSJ138.
- Figure 10 shows the DNA sequence (SEQ. ID. NO. 16), restriction map and predicted open reading frame (including predicted amino acid sequence, SEQ. ID. NO. 17) of a pullulanase type partial length potato debranching enzyme cDNA clone (pSJ98, described below); nucleotides 46-774 are DBE, the other sequence is derived from pT7Blue vector;
- Figure 11 is a schematic representation of a "double" plant transformation vector, comprising partial isoamylase- and pullulanase-type debranching enzyme sequences operably linked in the anti-sense orientation to separate GBSS promoters;
- Figure 12 shows a zymogram (using a 5% polyacrylamide gel containing 1 % amylopectin) of mber extracts from potato lines transformed with an antisense isoamylase construct in accordance with the invention.
- Figure 13 shows viscosity (RVA) profiles of starch from potato lines transformed with an antisense isoamylase construct, and from a wild type control (1302).
- Example 1 Isolation of a partial cDNA clone of a potato isoamylase.
- PDBE2 ATC AAA TCT/G A/GAA nCC ATC DBE1 ATG GAT GTT GT(T/C/A) TA/TC/T AAT/C CAT
- Genomic DNA was isolated from potato leaves using a modified CTAB (cetyltrimethyl ammonium bromide) method (Murray and Thompson, 1980).
- Leaf tissue (4 gm) was frozen in liquid nitrogen and ground in a mortar and pestle and transferred to a 50 ml polypropylene centrifuge tube (Falcon) to which was added 40 ml of extraction buffer ( 2% w/v CTAB, 100 mM Tris-HCl pH 8.0, 1.4 M NaCl, 10 mM 3-mercaptoefhanol and 20 mM EDTA) and incubated at 65°C for 30 min.
- extraction buffer 2% w/v CTAB, 100 mM Tris-HCl pH 8.0, 1.4 M NaCl, 10 mM 3-mercaptoefhanol and 20 mM EDTA
- the mixture was split into two tubes and 15 ml of chloroform was added to each followed by gentle (end over end) mixing for 15 min at room temperature.
- the tubes were then centrifuged for 5 min at 5000g and the pooled aqueous phases were re-extracted with 5 ml of chloroform.
- the DNA was then precipitated by incubating on ice for 30 minutes with an equal volume of isopropanol and centrifuged for 5 minutes at 3000g. The pellet was washed once with 70% ethanol, briefly air-dried and resuspended in TE pH 8.0 containing 20 ⁇ g ml RNase A.
- the DNA was extracted with 5ml of chloroform, one tenth volume of 3 M sodium acetate (NaAc) pH 5.2 was added and the DNA precipitated with two volumes of ethanol. After centrifugation and washing as above, the DNA was resuspended in 2 ml of TE buffer at pH 8.0 and estimated to be approximately 150 ng/ ⁇ l by fluorometric measurement.
- oligonucleotide primers were made corresponding to these sequences (DBE2 & DBE1 respectively, see above), taking into consideration the codon usage in plant genes in order to reduce the degeneracy of the primers. These were used in a PCR reaction using first strand cDNA from leaf or tuber RNA or genomic DNA as template.
- Colonies were screened by PCR and positives were grown up and DNA isolated from a 50 ml culture by alkaline lysis and PEG precipitation. One clone of each of the 5 PCR fragments was sequenced from either end and in one direction only, therefore the sequence cannot be guaranteed 100 % correct.
- the exon sequences of the 1.8 and 1.6 kb bands (clones #43 & #34, designated pSJ134 and pSJ135 respectively) were found to be identical to pSJ98 (a pullulanase type DBE partial clone, described below) indicating that they are pullulanase type debranching enzymes.
- the two clones pSJ134 and pSJ135 are not identical, sharing 96.3% identity over the entire sequence at the 5' end, and 89.8% identity over 160 nucleotides at the 3' end, after which the two sequences diverge.
- Figure 2 A shows a comparison of the sequences of clones 134 and 135 at their 5' ends
- Figure 2B shows a comparison of their sequences at the 3' end
- Figures 3 A and 3B illustrate the presumed intron exon structure of the 5' and 3' ends (respectively) of clone 134
- Figures 4A and 4B illustrate the presumed intron exon structure of the 5' and 3' ends respectively of clone 135.
- two exons can be distinguished, separated by a 101 bp intron and the remainder of the exon sequences are present in the 3 ' end.
- FIG. 1 shows a comparison of the 5' ends of these clones, whilst Figure 5B compares their 3' ends. As with the pullulanase clones, clones 133 and 137 were not identical (96 and 97% identity at the 5' and 3' ends respectively).
- Figures 6A and 6B illustrate the presumed intron/exon structure of the 5' and 3' ends (respectively) of clone 133.
- Figures 7A and 7B illustrate the presumed intron/exon structure of the 5' and 3' ends respectively of clone 137. Only one intron can be distinguished at each end.
- the 0.8 kb clone although it had the correct primers on both ends was not a debranching enzyme but had homology to a rice Expressed Sequence Tag (RICR2232A) which encodes a 70kDa endomembrane protein.
- a new sense primer (PIAl) was designed to the isoamylase sequences just downstream of the DBE1 primer and this was use in a 3 'RACE PCR to clone the 3' end of the isoamylase gene.
- Five ⁇ l of first strand cDNA and 25 pmol of each primer (PIAl and Ro) were used in a 50 ⁇ l PCR reaction (94°C 2 min followed by 30 cycles of 94°C 45 seconds, 55°C 25 seconds, 72°C 2 min).
- One ⁇ l of this reaction was then used in a second PCR amplification using- PIAl and R, primers.
- a major band of approx 1.5 kb (the expected size) was amplified from both leaf and mber cDNA templates.
- the mber PCR fragment was purified on a 1% TAE agarose gel and then cloned in pT7Blue (clone designated pSJ132), The sequence and predicted ORF of this partial length clone is shown in Figure 8.
- the ORF contains 429 aa and is 77.0% identical to the maize isoamylase protein encoded by the sugary- 1 gene (data not shown).
- a plant transformation vector with the hygromycin selectable marker was constructed in which the 1.5 kb isoamylase cDNA from plasmid pSJ132 was inserted in an antisense orientation under the control of the granule bound starch synthase (GBSS) promoter. This vector (pSJ138) was then used to transform wild type and waxy potato plants.
- GBSS granule bound starch synthase
- a plant transformation vector (pSJ138) was constructed by inserting the 1.5 kb isoamylase cDNA from plasmid pSJ132 (as an Xba l-Sac I fragment) in an antisense orientation under the control of the granule bound starch synthase (GBSS) promoter into the plant transformation vector pSJ39 which had been cut with the same enzymes.
- GBSS granule bound starch synthase
- pSJ39 is a modified GPTV-HYG vector (Becker et al 1992) containing a 0.8 kb GBSS promoter and was constructed as follows: first the BamHI site between the hygromycin selectable marker and the gene 7 polyadenylation signal in pGPTV-HYG was destroyed by cutting and filling in with klenow polymerase to create pSJ35.
- Hfndlll-EcoRI fragment of this vector (containing the GUS gene & nos polyadenylation signal) was replaced with the H dlll- EcoRI fragment (containing the GBSS promoter-GUS-nos poly(A) cassette) from plasmid pGB121 (obtained from R Visser, Wageningen) to create plasmid pSJ39.
- Transformed potato plants cv Desiree were produced by co-cultivation of explants with Agrobacterium tumefaciens LBA 4404 containing plant transformation vector plasmids. Two explant types were used, microtubers and leaf fragments, both produced from stock cultures, requiring different methods in culture. Stocks of plantlets were maintained of wild type untransformed Desiree or selected lines previously transformed with antisense genes coding for enzymes of the starch biosynthetic pathway by regular transfer of single node explants using Murashige and Skoog media (1962 Physiol. Plant 15, 473) solidified with 0.8% agar with the sucrose concentration reduced from 3% to 1%.
- Microtuber explants were produced by transferring nodes to the same medium with the sucrose concentration increased to 8% plus the addition of 2.5mg/l benzylaminopurine. All cultures were grown at 22°C with illumination from fluorescent tubes at 40 ⁇ E/sqm for 16 hours, except those cltures grown to produce microtubers, which were kept in darkness.
- the protocol for microtuber explants, and that for leaf explants are detailed below.
- Explants were initially precultured on the appropriate multiplication medium for two days before co-cultivation. Co-cultivation was for 10 minutes in an overnight culture of the Agrobacterium before explants were blotted on filter paper to remove excess bacteria. After blotting explants were transferred to filter paper on feeder layers and incubated in darkness at 22°C for two days.
- the feeder layers consisted of a 9cm petri dish containing the appropriate multiplication media covered by 2ml of stationary phase tobacco suspension culture cells which were in turn covered by two layers filter paper.
- Tissue culture and selection using microtuber explants was performed as follows. Microtubers were taken from stock when they were sufficiently large and mature to use. This occurred after 6-8 weeks from transfer of nodes to the high sucrose medium when they weighed more than 30mg and had started to turn from white to pink. Stocks were used for several months after mber initiation provided they remained dormant and no new shoots or stolons had emerged from the bud end. Each tuber was cut in half longitudinally through the terminal bud and the cut end put in contact with the medium.
- the multiplication medium (Z5) used contained the salts and vitamins of Murashige and Skoog plus 3% sucrose, 0.2mg/l indole acetic acid, 5 mg/1 zeatin and 0.8% agar.
- Kanamycin was added at lOOmg/1 when NPTII was used and hygromycin at 15mg/l when HPTII was used.
- the explants were transferred to fresh medium every two weeks. Explants had two transfers on medium Z5, followed by one transfer on the same medium, except indole acetic acid and zeatin were replaced by lOmg/1 of gibberellic acid (medium MSG). At each transfer all shoots were removed and discarded. The final transfer was to hormone- free Murashige and Skoog medium with the sucrose concentration reduced from 3% to 1%. Shoots were allowed to develop at this stage. Tissue culture and selection using leaf explants was performed as follows.
- the multiplication medium used contained the salts and vitamins of Murashige and Skoog medium " plus 1.6% glucose, 0.02 mg/1 naphthyl acetic acid, 2mg/l zeatin * riboside and 0.8% agar. Kanamycin was added at lOOmg/1 when NPTII was used and hygromycin at 5mg/l when HPTII was used. Cultures were transferred every two weeks to the same medium. After four transfers the cultures which were vitrified or showed poor shoot development were transferred to liquid Murashige and Skoog medium containing 1.6% sucrose only.
- Transformation was confirmed as follows. Shoots which developed from either mber of leaf explants after the multiplication/organogenesis phase were transferred individually to hormone-free Murashige and Skoog medium containing 1% sucrose and 100 mg/1 kanamycin or 15mg/l hygromycin depending on the selection which was used. Only one shoot was taken from each explant unless the origin of separate shoots were well separated on the explant. Shoots which rooted on selection within two weeks were transferred to the same medium without selection and given an identifying number. When growth allowed sufficient tissue to be sampled, DNA was extracted from a few mgs of leaf and stem tissue of the culture using the method published by Edwards et al (1991 Nucleic Acids Research 19,(6) 1349) and transformation was confirmed by PCR using gene specific primers. Material was left in culture to allow individual shoots to be micropropagated. Positive shoots were micropropagated to be stored in vitro and also to provide planting material.
- Representative samples of the mbers were stored for analysis and .possible regrowth and' 150-200g fresh weight was taken for starch extraction.
- Washed mbers were diced and homogenised to a juice by passage through a Braun MP 75 centrifugal juicer.
- the juice was diluted with tap water to a volume of approximately 3L in a 5L conical flask. After 30 minutes the unsedimented supernatant liquid was discarded and the remaining solids washed again with a further 3L of water. After a further 30 minutes the liquid was decanted and discarded.
- the slurry of solids was poured through a 500 ⁇ m metal mesh filter which retained the bulk of the cell debris.
- the crude starch which passed through the filter was washed in the same manner until no protein foam remained and only white starch settled on the base of the flask below a clear supernatant liquid.
- the starch was harvested on filter paper using a Buchner funnel and washed on the funnel with two 500ml volumes of distiled water. Finally the starch was washed with 500ml of acetone and dried in a fume hood for approximately 30 minutes
- the degree of branching of the starches was determined by ⁇ NMR spectroscopy (Gidley
- Example 3 Cloning of a pullulanase type debranching enzyme from potato.
- a PCR based cloning strategy was devised for isolating pullulanase type debranching enzymes from potato, using conserved domains within the known cloned gene sequences, of which spinach was the only plant gene (genbank SOPULSPO_l).
- the amino acid sequence DGFRFDLMG was almost completely conserved (data not shown) and this is found at aa 546-554 of the spinach protein (964 aa in total). It was hoped therefore that this would also be present in the potato gene, as the sequence DGFRFD is also completely conserved in plant starch branching enzyme genes which belong to the same superfamily of amylolytic genes.
- Peptide sequences from the purified potato debranching enzyme which were published in WO 95/04826 were also compared to the spinach sequence; of the 12 peptide sequences 10 made possible matches to the spinach sequence and of these peptide number 1 (RTLLVNLDSDDVKPEGGDNL. corresponding to aa 300-319 of the-spinach sequence,' identical residues underlined) was chosen to make a degenerate oligonucleotide sense primer (PDBEl ;20mer,64x) to be used in PCR with a degenerate antisense primer designed to the sequence DGFRFD (PDBE2; 18mer, 16x). These primers were used in a PCR reaction with mber or leaf first strand cDNA as template.
- cDNA Five ⁇ l of cDNA was used as template in a 50 ⁇ l PCR reaction with 50 pmol of each degenerate primer (PDBEl & 2) under standard PCR conditions (28 cycles of 94°C 45 seconds, 48°C 25 seconds, 72°C 90 seconds).
- the PCR products were separated on a 1% agarose gel in TAE buffer.
- a strong band of the expected size (672bp) was amplified from the mber cDNA and a much weaker band from the leaf cDNA.
- the mber amplified band was cut out and purified by binding to DEAE paper and then cloned in the pT7Blue vector (Novagen).
- a diagram of the resulting vector is shown in Figure 11. In Figure 11, the symbols used have the same meanings as in Figure 9: pA-CaMV is the CaMV polyadenylation signal. This vector may then be used to transform potato plants, substantially as described in Example 2.
- zymograms are usually used to determine the presence or absence of debranching enzymes. Protein extracts were separated on native 5% polyacrylamide gels containing 1% potato amylopectin. After separation the gel was incubated for 1-3 hours in 100 mM citrate buffer pH 5.5 containing 10 mM EDTA, 1 mM acarbose, and 2.5 mM DTT. The gel was then rinsed twice in deionized water and stained with iodine (Lugol's solution). Debranching enzymes stain as blue bands against a purple background, whereas amylases appear as clear bands.
- the debranching enzyme bands in the amylopectin gel can thus be classified into isoamylase bands (which do not appear in the red pullulan containing gel) and pullulanase bands (which give clear bands in red pullulan containing gels).
- RVA Rotary Visco Analyser
- Viscosity development of starches was analysed using a Rapid Visco Analyser Series 4 instrument (Newport Scientific, Sydney, Australia). For the 13 minute profile, 2 g of starch was weighed into a sample cup and 25 ml of water was added to give a final starch concentration of 7.4 % (w/v) and the analysis was performed using the standard stirring and heating protocol according to the manufacturer's instructions. Viscosity was measured in centipoise (cP).
- Figure 13 shows the viscosity profiles of tuber starches from plants transformed with the antisense isoamylase construct.
- the linear plot shows the temperature profile.
- a wild type control line 1302, unbroken thin line
- Starch from line 1352 solid thick curve
- Starch from line 1352 shows a clearly reduced peak viscosity and a significantly higher setback viscosity compared to the wild type control (1302) and starches of transformed plants with no change in isoamylase expression level
- lines 1328 - thin dotted curve; 1365 - thin dotted/dashed line; and 1369 - thin dashed line shows a clearly reduced peak viscosity and a significantly higher setback viscosity compared to the wild type control (1302) and starches of transformed plants with no change in isoamylase expression level (lines 1328 - thin dotted curve; 1365 - thin dotted/dashed line; and 1369 - thin dashed line).
- the viscosity onset temperature is slightly increased for line
- the latter PCR fragment (492 bp) was isolated using primers derived from the sequence of a genomic clone which hybridised to a degenerate oligonucleotide very similar to the PDBEl oligo which was used in the experiments described here.
- the deduced amino acid sequence of the PCR fragment did not correspond exactly to the two peptide sequences from the purified protein (RTLLVNLDSDDVKPEGGDNL, SEQ. ID. NO. 30; and RLSSAGITHVHLLPTYNFA, SEQ. ID. NO. 31; differences are shown underlined).
- the inventors have also cloned two PCR fragments (2.4 and 2.0 kb; pSJ133 and 137 respectively) from genomic DNA of potato which have significant homology to isoamylase genes. These clones differ in the size, and perhaps number, of introns and are about 96-97% identical ( Figure 4), suggesting that they are derived from different genes.
- a cDNA fragment of approx 1500 bp was obtained by 3 'RACE; this cDNA encodes 429 aa which are 77% identical to the maize isoamylase gene and the inventors are confident that this gene is an isoamylase type DBE.
- the two different isoamylase-type DBE genomic sequences isolated by the inventors relate to two different genes having slightly different functions or roles in the potato plant, and that similar multiple isoamylase-type genes exist in other plants. Different isoamylase-type genes might, for instance, be subjected- to different regulatory * controls. As the genomic sequences recovered are not full length genes, it is possible that the respective gene sequences may diverge in the uncloned portions.
- pullulanase and isoamylase genes appear to be expressed in mbers and leaves since PCR bands of the correct size can be amplified from either mber or leaf first strand cDNA templates. The intensity of the amplified band is stronger in mbers for both DBE, possibly indicating that the expression level of both genes is higher in mbers, but this should be confirmed by northern analysis.
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002302639A CA2302639A1 (en) | 1997-09-06 | 1998-09-04 | Improvements in or relating to stability of plant starches |
AU89911/98A AU8991198A (en) | 1997-09-06 | 1998-09-04 | Improvements in or relating to stability of plant starches |
EP98941593A EP1009751A2 (en) | 1997-09-06 | 1998-09-04 | Improvements in or relating to stability of plant starches |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9718863.5 | 1997-09-06 | ||
GBGB9718863.5A GB9718863D0 (en) | 1997-09-06 | 1997-09-06 | Improvements in or relating to stability of plant starches |
Publications (3)
Publication Number | Publication Date |
---|---|
WO1999012950A2 true WO1999012950A2 (en) | 1999-03-18 |
WO1999012950A3 WO1999012950A3 (en) | 1999-05-06 |
WO1999012950A9 WO1999012950A9 (en) | 1999-06-10 |
Family
ID=10818600
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB1998/002665 WO1999012950A2 (en) | 1997-09-06 | 1998-09-04 | Improvements in or relating to stability of plant starches |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1009751A2 (en) |
AU (1) | AU8991198A (en) |
CA (1) | CA2302639A1 (en) |
GB (1) | GB9718863D0 (en) |
WO (1) | WO1999012950A2 (en) |
Cited By (173)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6469230B1 (en) | 1997-07-31 | 2002-10-22 | Plant Bioscience Limited | Starch debranching enzymes |
EP2039771A2 (en) | 2009-01-06 | 2009-03-25 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants |
EP2039772A2 (en) | 2009-01-06 | 2009-03-25 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants introduction |
EP2039770A2 (en) | 2009-01-06 | 2009-03-25 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants |
EP2072506A1 (en) | 2007-12-21 | 2009-06-24 | Bayer CropScience AG | Thiazolyloxyphenylamidine or thiadiazolyloxyphenylamidine und its use as fungicide |
EP2090168A1 (en) | 2008-02-12 | 2009-08-19 | Bayer CropScience AG | Method for improving plant growth |
EP2168434A1 (en) | 2008-08-02 | 2010-03-31 | Bayer CropScience AG | Use of azols to increase resistance of plants of parts of plants to abiotic stress |
EP2198709A1 (en) | 2008-12-19 | 2010-06-23 | Bayer CropScience AG | Method for treating resistant animal pests |
EP2201838A1 (en) | 2008-12-05 | 2010-06-30 | Bayer CropScience AG | Active ingredient-beneficial organism combinations with insecticide and acaricide properties |
EP2204094A1 (en) | 2008-12-29 | 2010-07-07 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants Introduction |
WO2010083955A2 (en) | 2009-01-23 | 2010-07-29 | Bayer Cropscience Aktiengesellschaft | Use of enaminocarboxylic compounds for fighting viruses transmitted by insects |
WO2010086095A1 (en) | 2009-01-29 | 2010-08-05 | Bayer Cropscience Ag | Method for improved utilization of the production potential of transgenic plants introduction |
WO2010086311A1 (en) | 2009-01-28 | 2010-08-05 | Bayer Cropscience Ag | Fungicide n-cycloalkyl-n-bicyclicmethylene-carboxamide derivatives |
EP2218717A1 (en) | 2009-02-17 | 2010-08-18 | Bayer CropScience AG | Fungicidal N-((HET)Arylethyl)thiocarboxamide derivatives |
WO2010094666A2 (en) | 2009-02-17 | 2010-08-26 | Bayer Cropscience Ag | Fungicidal n-(phenylcycloalkyl)carboxamide, n-(benzylcycloalkyl)carboxamide and thiocarboxamide derivatives |
WO2010094728A1 (en) | 2009-02-19 | 2010-08-26 | Bayer Cropscience Ag | Pesticide composition comprising a tetrazolyloxime derivative and a fungicide or an insecticide active substance |
EP2223602A1 (en) | 2009-02-23 | 2010-09-01 | Bayer CropScience AG | Method for improved utilisation of the production potential of genetically modified plants |
EP2232995A1 (en) | 2009-03-25 | 2010-09-29 | Bayer CropScience AG | Method for improved utilisation of the production potential of transgenic plants |
EP2239331A1 (en) | 2009-04-07 | 2010-10-13 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants |
EP2251331A1 (en) | 2009-05-15 | 2010-11-17 | Bayer CropScience AG | Fungicide pyrazole carboxamides derivatives |
EP2255626A1 (en) | 2009-05-27 | 2010-12-01 | Bayer CropScience AG | Use of succinate dehydrogenase inhibitors to increase resistance of plants or parts of plants to abiotic stress |
WO2011006603A2 (en) | 2009-07-16 | 2011-01-20 | Bayer Cropscience Ag | Synergistic active substance combinations containing phenyl triazoles |
WO2011015524A2 (en) | 2009-08-03 | 2011-02-10 | Bayer Cropscience Ag | Fungicide heterocycles derivatives |
EP2292094A1 (en) | 2009-09-02 | 2011-03-09 | Bayer CropScience AG | Active compound combinations |
WO2011080256A1 (en) | 2009-12-28 | 2011-07-07 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
WO2011080254A2 (en) | 2009-12-28 | 2011-07-07 | Bayer Cropscience Ag | Fungicide hydroximoyl-heterocycles derivatives |
WO2011080255A2 (en) | 2009-12-28 | 2011-07-07 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
EP2343280A1 (en) | 2009-12-10 | 2011-07-13 | Bayer CropScience AG | Fungicide quinoline derivatives |
WO2011089071A2 (en) | 2010-01-22 | 2011-07-28 | Bayer Cropscience Ag | Acaricide and/or insecticide active substance combinations |
WO2011107504A1 (en) | 2010-03-04 | 2011-09-09 | Bayer Cropscience Ag | Fluoroalkyl-substituted 2-amidobenzimidazoles and the use thereof for boosting stress tolerance in plants |
EP2374791A1 (en) | 2008-08-14 | 2011-10-12 | Bayer CropScience Aktiengesellschaft | Insecticidal 4-phenyl-1H pyrazoles |
WO2011124554A2 (en) | 2010-04-06 | 2011-10-13 | Bayer Cropscience Ag | Use of 4-phenylbutyric acid and/or the salts thereof for enhancing the stress tolerance of plants |
WO2011124553A2 (en) | 2010-04-09 | 2011-10-13 | Bayer Cropscience Ag | Use of derivatives of the (1-cyanocyclopropyl)phenylphosphinic acid, the esters thereof and/or the salts thereof for enhancing the tolerance of plants to abiotic stress |
WO2011134912A1 (en) | 2010-04-28 | 2011-11-03 | Bayer Cropscience Ag | Fungicide hydroximoyl-heterocycles derivatives |
WO2011134913A1 (en) | 2010-04-28 | 2011-11-03 | Bayer Cropscience Ag | Fungicide hydroximoyl-heterocycles derivatives |
WO2011134911A2 (en) | 2010-04-28 | 2011-11-03 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
WO2011151369A1 (en) | 2010-06-03 | 2011-12-08 | Bayer Cropscience Ag | N-[(het)arylethyl)] pyrazole(thio)carboxamides and their heterosubstituted analogues |
WO2011151370A1 (en) | 2010-06-03 | 2011-12-08 | Bayer Cropscience Ag | N-[(het)arylalkyl)] pyrazole (thio)carboxamides and their heterosubstituted analogues |
WO2011151368A2 (en) | 2010-06-03 | 2011-12-08 | Bayer Cropscience Ag | Fungicide n-[(trisubstitutedsilyl)methyl]-carboxamide derivatives |
WO2011154158A1 (en) | 2010-06-09 | 2011-12-15 | Bayer Bioscience N.V. | Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering |
WO2011154159A1 (en) | 2010-06-09 | 2011-12-15 | Bayer Bioscience N.V. | Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering |
US8080688B2 (en) | 2007-03-12 | 2011-12-20 | Bayer Cropscience Ag | 3, 4-disubstituted phenoxyphenylamidines and use thereof as fungicides |
WO2012010579A2 (en) | 2010-07-20 | 2012-01-26 | Bayer Cropscience Ag | Benzocycloalkenes as antifungal agents |
WO2012028578A1 (en) | 2010-09-03 | 2012-03-08 | Bayer Cropscience Ag | Substituted fused pyrimidinones and dihydropyrimidinones |
WO2012038480A2 (en) | 2010-09-22 | 2012-03-29 | Bayer Cropscience Ag | Use of biological or chemical control agents for controlling insects and nematodes in resistant crops |
WO2012045798A1 (en) | 2010-10-07 | 2012-04-12 | Bayer Cropscience Ag | Fungicide composition comprising a tetrazolyloxime derivative and a thiazolylpiperidine derivative |
WO2012052489A1 (en) | 2010-10-21 | 2012-04-26 | Bayer Cropscience Ag | 1-(heterocyclic carbonyl) piperidines |
WO2012052490A1 (en) | 2010-10-21 | 2012-04-26 | Bayer Cropscience Ag | N-benzyl heterocyclic carboxamides |
US8168567B2 (en) | 2007-04-19 | 2012-05-01 | Bayer Cropscience Ag | Thiadiazolyl oxyphenyl amidines and the use thereof as a fungicide |
WO2012059497A1 (en) | 2010-11-02 | 2012-05-10 | Bayer Cropscience Ag | N-hetarylmethyl pyrazolylcarboxamides |
WO2012065944A1 (en) | 2010-11-15 | 2012-05-24 | Bayer Cropscience Ag | N-aryl pyrazole(thio)carboxamides |
WO2012065945A1 (en) | 2010-11-15 | 2012-05-24 | Bayer Cropscience Ag | 5-halogenopyrazole(thio)carboxamides |
WO2012065947A1 (en) | 2010-11-15 | 2012-05-24 | Bayer Cropscience Ag | 5-halogenopyrazolecarboxamides |
EP2460407A1 (en) | 2010-12-01 | 2012-06-06 | Bayer CropScience AG | Agent combinations comprising pyridylethyl benzamides and other agents |
EP2460406A1 (en) | 2010-12-01 | 2012-06-06 | Bayer CropScience AG | Use of fluopyram for controlling nematodes in nematode resistant crops |
WO2012072660A1 (en) | 2010-12-01 | 2012-06-07 | Bayer Cropscience Ag | Use of fluopyram for controlling nematodes in crops and for increasing yield |
WO2012089722A2 (en) | 2010-12-30 | 2012-07-05 | Bayer Cropscience Ag | Use of open-chain carboxylic acids, carbonic esters, carboxamides and carbonitriles of aryl, heteroaryl and benzylsulfonamide or the salts thereof for improving the stress tolerance in plants |
WO2012089757A1 (en) | 2010-12-29 | 2012-07-05 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
EP2474542A1 (en) | 2010-12-29 | 2012-07-11 | Bayer CropScience AG | Fungicide hydroximoyl-tetrazole derivatives |
EP2494867A1 (en) | 2011-03-01 | 2012-09-05 | Bayer CropScience AG | Halogen-substituted compounds in combination with fungicides |
WO2012120105A1 (en) | 2011-03-10 | 2012-09-13 | Bayer Cropscience Ag | Use of lipochito-oligosaccharide compounds for safeguarding seed safety of treated seeds |
WO2012123434A1 (en) | 2011-03-14 | 2012-09-20 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
WO2012136581A1 (en) | 2011-04-08 | 2012-10-11 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
US8288426B2 (en) | 2006-12-22 | 2012-10-16 | Bayer Cropscience Ag | Pesticidal composition comprising fenamidone and an insecticide compound |
EP2511255A1 (en) | 2011-04-15 | 2012-10-17 | Bayer CropScience AG | Substituted prop-2-in-1-ol and prop-2-en-1-ol derivatives |
WO2012139891A1 (en) | 2011-04-15 | 2012-10-18 | Bayer Cropscience Ag | Substituted vinyl and alkinyl cyclohexenols as active agents against abiotic stress in plants |
WO2012139892A1 (en) | 2011-04-15 | 2012-10-18 | Bayer Cropscience Ag | Substituted 5-(bicyclo[4.1.0]hept-3-en-2-yl)-penta-2,4-dienes and 5-(bicyclo[4.1.0]hept-3-en-2-yl)-pent-2-ene-4-ines as active agents against abiotic stress in plants |
WO2012139890A1 (en) | 2011-04-15 | 2012-10-18 | Bayer Cropscience Ag | Substituted 5-(cyclohex-2-en-1-yl)-penta-2,4-dienes and 5-(cyclohex-2-en-1-yl)-pent-2-en-4-ines as active agents against abiotic stress in plants |
US8299302B2 (en) | 2007-03-12 | 2012-10-30 | Bayer Cropscience Ag | 4-Cycloalkyl or 4-substituted phenoxyphenylamidines and use thereof as fungicides |
WO2012168124A1 (en) | 2011-06-06 | 2012-12-13 | Bayer Cropscience Nv | Methods and means to modify a plant genome at a preselected site |
WO2013004652A1 (en) | 2011-07-04 | 2013-01-10 | Bayer Intellectual Property Gmbh | Use of substituted isoquinolinones, isoquinolindiones, isoquinolintriones and dihydroisoquinolinones or in each case salts thereof as active agents against abiotic stress in plants |
WO2013020985A1 (en) | 2011-08-10 | 2013-02-14 | Bayer Intellectual Property Gmbh | Active compound combinations comprising specific tetramic acid derivatives |
EP2561759A1 (en) | 2011-08-26 | 2013-02-27 | Bayer Cropscience AG | Fluoroalkyl-substituted 2-amidobenzimidazoles and their effect on plant growth |
WO2013026836A1 (en) | 2011-08-22 | 2013-02-28 | Bayer Intellectual Property Gmbh | Fungicide hydroximoyl-tetrazole derivatives |
WO2013026740A2 (en) | 2011-08-22 | 2013-02-28 | Bayer Cropscience Nv | Methods and means to modify a plant genome |
US8394991B2 (en) | 2007-03-12 | 2013-03-12 | Bayer Cropscience Ag | Phenoxy substituted phenylamidine derivatives and their use as fungicides |
WO2013034621A1 (en) | 2011-09-09 | 2013-03-14 | Bayer Intellectual Property Gmbh | Acyl-homoserine lactone derivatives for improving plant yield |
WO2013037956A1 (en) | 2011-09-16 | 2013-03-21 | Bayer Intellectual Property Gmbh | Use of 5-phenyl- or 5-benzyl-2 isoxazoline-3 carboxylates for improving plant yield |
WO2013037958A1 (en) | 2011-09-16 | 2013-03-21 | Bayer Intellectual Property Gmbh | Use of phenylpyrazolin-3-carboxylates for improving plant yield |
WO2013037955A1 (en) | 2011-09-16 | 2013-03-21 | Bayer Intellectual Property Gmbh | Use of acylsulfonamides for improving plant yield |
WO2013037717A1 (en) | 2011-09-12 | 2013-03-21 | Bayer Intellectual Property Gmbh | Fungicidal 4-substituted-3-{phenyl[(heterocyclylmethoxy)imino]methyl}-1,2,4-oxadizol-5(4h)-one derivatives |
WO2013041602A1 (en) | 2011-09-23 | 2013-03-28 | Bayer Intellectual Property Gmbh | Use of 4-substituted 1-phenyl-pyrazole-3-carboxylic-acid derivatives as agents against abiotic plant stress |
WO2013050410A1 (en) | 2011-10-04 | 2013-04-11 | Bayer Intellectual Property Gmbh | RNAi FOR THE CONTROL OF FUNGI AND OOMYCETES BY INHIBITING SACCHAROPINE DEHYDROGENASE GENE |
WO2013050324A1 (en) | 2011-10-06 | 2013-04-11 | Bayer Intellectual Property Gmbh | Combination, containing 4-phenylbutyric acid (4-pba) or a salt thereof (component (a)) and one or more selected additional agronomically active compounds (component(s) (b)), that reduces abiotic plant stress |
WO2013075817A1 (en) | 2011-11-21 | 2013-05-30 | Bayer Intellectual Property Gmbh | Fungicide n-[(trisubstitutedsilyl)methyl]-carboxamide derivatives |
US8455480B2 (en) | 2007-09-26 | 2013-06-04 | Bayer Cropscience Ag | Active agent combinations having insecticidal and acaricidal properties |
WO2013079566A2 (en) | 2011-11-30 | 2013-06-06 | Bayer Intellectual Property Gmbh | Fungicidal n-bicycloalkyl and n-tricycloalkyl (thio)carboxamide derivatives |
WO2013092519A1 (en) | 2011-12-19 | 2013-06-27 | Bayer Cropscience Ag | Use of anthranilic acid diamide derivatives for pest control in transgenic crops |
WO2013098146A1 (en) | 2011-12-29 | 2013-07-04 | Bayer Intellectual Property Gmbh | Fungicidal 3-[(1,3-thiazol-4-ylmethoxyimino)(phenyl)methyl]-2-substituted-1,2,4-oxadiazol-5(2h)-one derivatives |
WO2013098147A1 (en) | 2011-12-29 | 2013-07-04 | Bayer Intellectual Property Gmbh | Fungicidal 3-[(pyridin-2-ylmethoxyimino)(phenyl)methyl]-2-substituted-1,2,4-oxadiazol-5(2h)-one derivatives |
US8487118B2 (en) | 2009-01-19 | 2013-07-16 | Bayer Cropscience Ag | Cyclic diones and their use as insecticides, acaricides and/or fungicides |
WO2013124275A1 (en) | 2012-02-22 | 2013-08-29 | Bayer Cropscience Ag | Use of succinate dehydrogenase inhibitors (sdhis) for controlling wood diseases in grape. |
WO2013127704A1 (en) | 2012-02-27 | 2013-09-06 | Bayer Intellectual Property Gmbh | Active compound combinations containing a thiazoylisoxazoline and a fungicide |
WO2013139949A1 (en) | 2012-03-23 | 2013-09-26 | Bayer Intellectual Property Gmbh | Compositions comprising a strigolactame compound for enhanced plant growth and yield |
WO2013153143A1 (en) | 2012-04-12 | 2013-10-17 | Bayer Cropscience Ag | N-acyl- 2 - (cyclo) alkylpyrrolidines and piperidines useful as fungicides |
WO2013156560A1 (en) | 2012-04-20 | 2013-10-24 | Bayer Cropscience Ag | N-cycloalkyl-n-[(trisubstitutedsilylphenyl)methylene]-(thio)carboxamide derivatives |
WO2013156559A1 (en) | 2012-04-20 | 2013-10-24 | Bayer Cropscience Ag | N-cycloalkyl-n-[(heterocyclylphenyl)methylene]-(thio)carboxamide derivatives |
WO2013160230A1 (en) | 2012-04-23 | 2013-10-31 | Bayer Cropscience Nv | Targeted genome engineering in plants |
EP2662361A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazol indanyl carboxamides |
EP2662364A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazole tetrahydronaphthyl carboxamides |
EP2662362A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazole indanyl carboxamides |
EP2662363A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole biphenylcarboxamides |
EP2662370A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole benzofuranyl carboxamides |
EP2662360A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole indanyl carboxamides |
WO2013167544A1 (en) | 2012-05-09 | 2013-11-14 | Bayer Cropscience Ag | 5-halogenopyrazole indanyl carboxamides |
WO2013167545A1 (en) | 2012-05-09 | 2013-11-14 | Bayer Cropscience Ag | Pyrazole indanyl carboxamides |
WO2013174836A1 (en) | 2012-05-22 | 2013-11-28 | Bayer Cropscience Ag | Active compounds combinations comprising a lipo-chitooligosaccharide derivative and a nematicide, insecticidal or fungicidal compound |
WO2014009322A1 (en) | 2012-07-11 | 2014-01-16 | Bayer Cropscience Ag | Use of fungicidal combinations for increasing the tolerance of a plant towards abiotic stress |
WO2014037340A1 (en) | 2012-09-05 | 2014-03-13 | Bayer Cropscience Ag | Use of substituted 2-amidobenzimidazoles, 2-amidobenzoxazoles and 2-amidobenzothiazoles or salts thereof as active substances against abiotic plant stress |
WO2014060520A1 (en) | 2012-10-19 | 2014-04-24 | Bayer Cropscience Ag | Method for treating plants against fungi resistant to fungicides using carboxamide or thiocarboxamide derivatives |
WO2014060502A1 (en) | 2012-10-19 | 2014-04-24 | Bayer Cropscience Ag | Active compound combinations comprising carboxamide derivatives |
WO2014060518A1 (en) | 2012-10-19 | 2014-04-24 | Bayer Cropscience Ag | Method of plant growth promotion using carboxamide derivatives |
WO2014060519A1 (en) | 2012-10-19 | 2014-04-24 | Bayer Cropscience Ag | Method for enhancing tolerance to abiotic stress in plants using carboxamide or thiocarboxamide derivatives |
EP2735231A1 (en) | 2012-11-23 | 2014-05-28 | Bayer CropScience AG | Active compound combinations |
WO2014079957A1 (en) | 2012-11-23 | 2014-05-30 | Bayer Cropscience Ag | Selective inhibition of ethylene signal transduction |
WO2014083089A1 (en) | 2012-11-30 | 2014-06-05 | Bayer Cropscience Ag | Ternary fungicidal and pesticidal mixtures |
WO2014083088A2 (en) | 2012-11-30 | 2014-06-05 | Bayer Cropscience Ag | Binary fungicidal mixtures |
WO2014082950A1 (en) | 2012-11-30 | 2014-06-05 | Bayer Cropscience Ag | Ternary fungicidal mixtures |
WO2014083033A1 (en) | 2012-11-30 | 2014-06-05 | Bayer Cropsience Ag | Binary fungicidal or pesticidal mixture |
WO2014083031A2 (en) | 2012-11-30 | 2014-06-05 | Bayer Cropscience Ag | Binary pesticidal and fungicidal mixtures |
EP2740356A1 (en) | 2012-12-05 | 2014-06-11 | Bayer CropScience AG | Substituted (2Z)-5(1-Hydroxycyclohexyl)pent-2-en-4-inic acid derivatives |
EP2740720A1 (en) | 2012-12-05 | 2014-06-11 | Bayer CropScience AG | Substituted bicyclic and tricyclic pent-2-en-4-inic acid derivatives and their use for enhancing the stress tolerance in plants |
WO2014086751A1 (en) | 2012-12-05 | 2014-06-12 | Bayer Cropscience Ag | Use of substituted 1-(aryl ethynyl)-, 1-(heteroaryl ethynyl)-, 1-(heterocyclyl ethynyl)- and 1-(cyloalkenyl ethynyl)-cyclohexanols as active agents against abiotic plant stress |
WO2014090765A1 (en) | 2012-12-12 | 2014-06-19 | Bayer Cropscience Ag | Use of 1-[2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfinyl)phenyl]-5-amino-3-trifluoromethyl)-1 h-1,2,4 tfia zole for controlling nematodes in nematode-resistant crops |
WO2014095677A1 (en) | 2012-12-19 | 2014-06-26 | Bayer Cropscience Ag | Difluoromethyl-nicotinic- tetrahydronaphtyl carboxamides |
WO2014095826A1 (en) | 2012-12-18 | 2014-06-26 | Bayer Cropscience Ag | Binary fungicidal and bactericidal combinations |
US8785692B2 (en) | 2007-03-12 | 2014-07-22 | Bayer Cropscience Ag | Substituted phenylamidines and the use thereof as fungicides |
US8796175B2 (en) | 2008-08-29 | 2014-08-05 | Bayer Cropscience Ag | Method for enhancing plant intrinsic defense |
US8828906B2 (en) | 2009-03-25 | 2014-09-09 | Bayer Cropscience Ag | Active compound combinations having insecticidal and acaricidal properties |
US8828907B2 (en) | 2009-03-25 | 2014-09-09 | Bayer Cropscience Ag | Active ingredient combinations having insecticidal and acaricidal properties |
WO2014135608A1 (en) | 2013-03-07 | 2014-09-12 | Bayer Cropscience Ag | Fungicidal 3-{phenyl[(heterocyclylmethoxy)imino]methyl}-heterocycle derivatives |
US8835657B2 (en) | 2009-05-06 | 2014-09-16 | Bayer Cropscience Ag | Cyclopentanedione compounds and their use as insecticides, acaricides and/or fungicides |
US8846567B2 (en) | 2009-03-25 | 2014-09-30 | Bayer Cropscience Ag | Active compound combinations having insecticidal and acaricidal properties |
US8846568B2 (en) | 2009-03-25 | 2014-09-30 | Bayer Cropscience Ag | Active compound combinations having insecticidal and acaricidal properties |
WO2014161821A1 (en) | 2013-04-02 | 2014-10-09 | Bayer Cropscience Nv | Targeted genome engineering in eukaryotes |
WO2014167009A1 (en) | 2013-04-12 | 2014-10-16 | Bayer Cropscience Ag | Novel triazole derivatives |
WO2014167008A1 (en) | 2013-04-12 | 2014-10-16 | Bayer Cropscience Ag | Novel triazolinthione derivatives |
WO2014170364A1 (en) | 2013-04-19 | 2014-10-23 | Bayer Cropscience Ag | Binary insecticidal or pesticidal mixture |
WO2014170345A2 (en) | 2013-04-19 | 2014-10-23 | Bayer Cropscience Ag | Method for improved utilization of the production potential of transgenic plants |
WO2014177514A1 (en) | 2013-04-30 | 2014-11-06 | Bayer Cropscience Ag | Nematicidal n-substituted phenethylcarboxamides |
WO2014177582A1 (en) | 2013-04-30 | 2014-11-06 | Bayer Cropscience Ag | N-(2-fluoro-2-phenethyl)carboxamides as nematicides and endoparasiticides |
WO2014206953A1 (en) | 2013-06-26 | 2014-12-31 | Bayer Cropscience Ag | N-cycloalkyl-n-[(bicyclylphenyl)methylene]-(thio)carboxamide derivatives |
US8927583B2 (en) | 2006-12-22 | 2015-01-06 | Bayer Cropscience Ag | Pesticidal composition comprising a 2-pyrdilmethylbenzamide derivative and an insecticide compound |
WO2015004040A1 (en) | 2013-07-09 | 2015-01-15 | Bayer Cropscience Ag | Use of selected pyridone carboxamides or salts thereof as active substances against abiotic plant stress |
US9012360B2 (en) | 2009-03-25 | 2015-04-21 | Bayer Intellectual Property Gmbh | Synergistic combinations of active ingredients |
WO2015082586A1 (en) | 2013-12-05 | 2015-06-11 | Bayer Cropscience Ag | N-cycloalkyl-n-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives |
WO2015082587A1 (en) | 2013-12-05 | 2015-06-11 | Bayer Cropscience Ag | N-cycloalkyl-n-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives |
US9199922B2 (en) | 2007-03-12 | 2015-12-01 | Bayer Intellectual Property Gmbh | Dihalophenoxyphenylamidines and use thereof as fungicides |
US9232794B2 (en) | 2009-06-02 | 2016-01-12 | Bayer Intellectual Property Gmbh | Use of succinate dehydrogenase inhibitors for controlling Sclerotinia ssp |
WO2016012362A1 (en) | 2014-07-22 | 2016-01-28 | Bayer Cropscience Aktiengesellschaft | Substituted cyano cycloalkyl penta-2,4-dienes, cyano cycloalkyl pent-2-en-4-ynes, cyano heterocyclyl penta-2,4-dienes and cyano heterocyclyl pent-2-en-4-ynes as active substances against abiotic plant stress |
EP2997825A1 (en) | 2011-04-22 | 2016-03-23 | Bayer Intellectual Property GmbH | Active compound combinations comprising a (thio)carboxamide derivative and a fungicidal compound |
EP3000809A1 (en) | 2009-05-15 | 2016-03-30 | Bayer Intellectual Property GmbH | Fungicide pyrazole carboxamides derivatives |
WO2016096942A1 (en) | 2014-12-18 | 2016-06-23 | Bayer Cropscience Aktiengesellschaft | Use of selected pyridone carboxamides or salts thereof as active substances against abiotic plant stress |
WO2016166077A1 (en) | 2015-04-13 | 2016-10-20 | Bayer Cropscience Aktiengesellschaft | N-cycloalkyl-n-(biheterocyclyethylene)-(thio)carboxamide derivatives |
US9763451B2 (en) | 2008-12-29 | 2017-09-19 | Bayer Intellectual Property Gmbh | Method for improved use of the production potential of genetically modified plants |
WO2018019676A1 (en) | 2016-07-29 | 2018-02-01 | Bayer Cropscience Aktiengesellschaft | Active compound combinations and methods to protect the propagation material of plants |
WO2018054911A1 (en) | 2016-09-23 | 2018-03-29 | Bayer Cropscience Nv | Targeted genome optimization in plants |
WO2018054832A1 (en) | 2016-09-22 | 2018-03-29 | Bayer Cropscience Aktiengesellschaft | Novel triazole derivatives |
WO2018054829A1 (en) | 2016-09-22 | 2018-03-29 | Bayer Cropscience Aktiengesellschaft | Novel triazole derivatives and their use as fungicides |
WO2018077711A2 (en) | 2016-10-26 | 2018-05-03 | Bayer Cropscience Aktiengesellschaft | Use of pyraziflumid for controlling sclerotinia spp in seed treatment applications |
EP3332645A1 (en) | 2016-12-12 | 2018-06-13 | Bayer Cropscience AG | Use of substituted pyrimidine diones or their salts as agents to combat abiotic plant stress |
WO2018104392A1 (en) | 2016-12-08 | 2018-06-14 | Bayer Cropscience Aktiengesellschaft | Use of insecticides for controlling wireworms |
WO2018108627A1 (en) | 2016-12-12 | 2018-06-21 | Bayer Cropscience Aktiengesellschaft | Use of substituted indolinylmethyl sulfonamides, or the salts thereof for increasing the stress tolerance of plants |
DE102007045953B4 (en) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Drug combinations with insecticidal and acaricidal properties |
DE102007045920B4 (en) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Synergistic drug combinations |
DE102007045919B4 (en) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Drug combinations with insecticidal and acaricidal properties |
WO2019025153A1 (en) | 2017-07-31 | 2019-02-07 | Bayer Cropscience Aktiengesellschaft | Use of substituted n-sulfonyl-n'-aryl diaminoalkanes and n-sulfonyl-n'-heteroaryl diaminoalkanes or salts thereof for increasing the stress tolerance in plants |
WO2019060746A1 (en) | 2017-09-21 | 2019-03-28 | The Broad Institute, Inc. | Systems, methods, and compositions for targeted nucleic acid editing |
WO2019233863A1 (en) | 2018-06-04 | 2019-12-12 | Bayer Aktiengesellschaft | Herbicidally active bicyclic benzoylpyrazoles |
WO2020131862A1 (en) | 2018-12-17 | 2020-06-25 | The Broad Institute, Inc. | Crispr-associated transposase systems and methods of use thereof |
US10968257B2 (en) | 2018-04-03 | 2021-04-06 | The Broad Institute, Inc. | Target recognition motifs and uses thereof |
US11180751B2 (en) | 2015-06-18 | 2021-11-23 | The Broad Institute, Inc. | CRISPR enzymes and systems |
US11591601B2 (en) | 2017-05-05 | 2023-02-28 | The Broad Institute, Inc. | Methods for identification and modification of lncRNA associated with target genotypes and phenotypes |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102007045922A1 (en) | 2007-09-26 | 2009-04-02 | Bayer Cropscience Ag | Drug combinations with insecticidal and acaricidal properties |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995004826A1 (en) * | 1993-08-09 | 1995-02-16 | Institut Für Genbiologische Forschung Berlin Gmbh | Debranching enzymes and dna sequences coding them, suitable for changing the degree of branching of amylopectin starch in plants |
WO1996003513A2 (en) * | 1994-07-28 | 1996-02-08 | Monsanto Company | Isoamylase gene from flaviobacterium sp., compositions containing it and methods using it |
WO1996019581A1 (en) * | 1994-12-22 | 1996-06-27 | Hoechst Schering Agrevo Gmbh | Dna molecules coding for debranching enzymes derived from plants |
WO1997042328A1 (en) * | 1996-05-06 | 1997-11-13 | Planttec Biotechnologie Gmbh | Nucleic acid molecules which code the potato debranching enzyme |
WO1999006575A1 (en) * | 1997-07-31 | 1999-02-11 | Plant Bioscience Limited | Starch debranching enzymes |
-
1997
- 1997-09-06 GB GBGB9718863.5A patent/GB9718863D0/en not_active Ceased
-
1998
- 1998-09-04 WO PCT/GB1998/002665 patent/WO1999012950A2/en not_active Application Discontinuation
- 1998-09-04 EP EP98941593A patent/EP1009751A2/en not_active Withdrawn
- 1998-09-04 CA CA002302639A patent/CA2302639A1/en not_active Abandoned
- 1998-09-04 AU AU89911/98A patent/AU8991198A/en not_active Abandoned
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995004826A1 (en) * | 1993-08-09 | 1995-02-16 | Institut Für Genbiologische Forschung Berlin Gmbh | Debranching enzymes and dna sequences coding them, suitable for changing the degree of branching of amylopectin starch in plants |
WO1996003513A2 (en) * | 1994-07-28 | 1996-02-08 | Monsanto Company | Isoamylase gene from flaviobacterium sp., compositions containing it and methods using it |
WO1996019581A1 (en) * | 1994-12-22 | 1996-06-27 | Hoechst Schering Agrevo Gmbh | Dna molecules coding for debranching enzymes derived from plants |
WO1997042328A1 (en) * | 1996-05-06 | 1997-11-13 | Planttec Biotechnologie Gmbh | Nucleic acid molecules which code the potato debranching enzyme |
WO1999006575A1 (en) * | 1997-07-31 | 1999-02-11 | Plant Bioscience Limited | Starch debranching enzymes |
Non-Patent Citations (3)
Title |
---|
ISHIZAKI Y ET AL: "DEBRANCHING ENZYMES OF POTATO TUBERS (SOLANUM TUBEROSUM L.) I PURIFICATION AND SOME PROPERTIES OF POTATO ISOMYLASE" AGRICULTURAL AND BIOLOGICAL CHEMISTRY, vol. 47, no. 4, 1 January 1983, pages 771-779, XP002001115 * |
KORTSTEE ET AL: "Expression of Escherichia coli branching enzyme in tubers of amylose-free transgenic potato leads to an increased branching degree of the amylopectin" PLANT JOURNAL, vol. 10, no. 1, 1996, pages 83-90, XP002084156 cited in the application * |
SHEWMAKER C K ET AL: "EXPRESSION OF ESCHERICHIA COLI GLYCOGEN SYNTHASE IN THE TUBERS OF TRANSGENIC POTATOES (SOLANUM TUBEROSUM) RESULTS IN A HIGHLY BRANCHED STARCH" PLANT PHYSIOLOGY, vol. 104, 1994, pages 1159-1166, XP002033871 * |
Cited By (192)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6469230B1 (en) | 1997-07-31 | 2002-10-22 | Plant Bioscience Limited | Starch debranching enzymes |
US8288426B2 (en) | 2006-12-22 | 2012-10-16 | Bayer Cropscience Ag | Pesticidal composition comprising fenamidone and an insecticide compound |
US8927583B2 (en) | 2006-12-22 | 2015-01-06 | Bayer Cropscience Ag | Pesticidal composition comprising a 2-pyrdilmethylbenzamide derivative and an insecticide compound |
US8299302B2 (en) | 2007-03-12 | 2012-10-30 | Bayer Cropscience Ag | 4-Cycloalkyl or 4-substituted phenoxyphenylamidines and use thereof as fungicides |
US8394991B2 (en) | 2007-03-12 | 2013-03-12 | Bayer Cropscience Ag | Phenoxy substituted phenylamidine derivatives and their use as fungicides |
US8080688B2 (en) | 2007-03-12 | 2011-12-20 | Bayer Cropscience Ag | 3, 4-disubstituted phenoxyphenylamidines and use thereof as fungicides |
US8785692B2 (en) | 2007-03-12 | 2014-07-22 | Bayer Cropscience Ag | Substituted phenylamidines and the use thereof as fungicides |
US8748662B2 (en) | 2007-03-12 | 2014-06-10 | Bayer Cropscience Ag | 4-cycloalkyl or 4-aryl substituted phenoxyphenylamidines and use thereof as fungicides |
US9199922B2 (en) | 2007-03-12 | 2015-12-01 | Bayer Intellectual Property Gmbh | Dihalophenoxyphenylamidines and use thereof as fungicides |
US8168567B2 (en) | 2007-04-19 | 2012-05-01 | Bayer Cropscience Ag | Thiadiazolyl oxyphenyl amidines and the use thereof as a fungicide |
DE102007045919B4 (en) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Drug combinations with insecticidal and acaricidal properties |
US8455480B2 (en) | 2007-09-26 | 2013-06-04 | Bayer Cropscience Ag | Active agent combinations having insecticidal and acaricidal properties |
DE102007045920B4 (en) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Synergistic drug combinations |
DE102007045953B4 (en) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Drug combinations with insecticidal and acaricidal properties |
EP2072506A1 (en) | 2007-12-21 | 2009-06-24 | Bayer CropScience AG | Thiazolyloxyphenylamidine or thiadiazolyloxyphenylamidine und its use as fungicide |
EP2090168A1 (en) | 2008-02-12 | 2009-08-19 | Bayer CropScience AG | Method for improving plant growth |
EP2168434A1 (en) | 2008-08-02 | 2010-03-31 | Bayer CropScience AG | Use of azols to increase resistance of plants of parts of plants to abiotic stress |
EP2374791A1 (en) | 2008-08-14 | 2011-10-12 | Bayer CropScience Aktiengesellschaft | Insecticidal 4-phenyl-1H pyrazoles |
US8796175B2 (en) | 2008-08-29 | 2014-08-05 | Bayer Cropscience Ag | Method for enhancing plant intrinsic defense |
EP2201838A1 (en) | 2008-12-05 | 2010-06-30 | Bayer CropScience AG | Active ingredient-beneficial organism combinations with insecticide and acaricide properties |
EP2198709A1 (en) | 2008-12-19 | 2010-06-23 | Bayer CropScience AG | Method for treating resistant animal pests |
WO2010075994A1 (en) | 2008-12-29 | 2010-07-08 | Bayer Cropscience Aktiengesellschaft | Treatment of transgenic crops with mixtures of fiproles and chloronicotinyls |
EP2204094A1 (en) | 2008-12-29 | 2010-07-07 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants Introduction |
US9763451B2 (en) | 2008-12-29 | 2017-09-19 | Bayer Intellectual Property Gmbh | Method for improved use of the production potential of genetically modified plants |
EP2039770A2 (en) | 2009-01-06 | 2009-03-25 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants |
EP2039772A2 (en) | 2009-01-06 | 2009-03-25 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants introduction |
EP2039771A2 (en) | 2009-01-06 | 2009-03-25 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants |
US8487118B2 (en) | 2009-01-19 | 2013-07-16 | Bayer Cropscience Ag | Cyclic diones and their use as insecticides, acaricides and/or fungicides |
EP2227951A1 (en) | 2009-01-23 | 2010-09-15 | Bayer CropScience AG | Application of enaminocarbonyl compounds for combating viruses transmitted by insects |
WO2010083955A2 (en) | 2009-01-23 | 2010-07-29 | Bayer Cropscience Aktiengesellschaft | Use of enaminocarboxylic compounds for fighting viruses transmitted by insects |
WO2010086311A1 (en) | 2009-01-28 | 2010-08-05 | Bayer Cropscience Ag | Fungicide n-cycloalkyl-n-bicyclicmethylene-carboxamide derivatives |
WO2010086095A1 (en) | 2009-01-29 | 2010-08-05 | Bayer Cropscience Ag | Method for improved utilization of the production potential of transgenic plants introduction |
EP2218717A1 (en) | 2009-02-17 | 2010-08-18 | Bayer CropScience AG | Fungicidal N-((HET)Arylethyl)thiocarboxamide derivatives |
WO2010094666A2 (en) | 2009-02-17 | 2010-08-26 | Bayer Cropscience Ag | Fungicidal n-(phenylcycloalkyl)carboxamide, n-(benzylcycloalkyl)carboxamide and thiocarboxamide derivatives |
WO2010094728A1 (en) | 2009-02-19 | 2010-08-26 | Bayer Cropscience Ag | Pesticide composition comprising a tetrazolyloxime derivative and a fungicide or an insecticide active substance |
EP2223602A1 (en) | 2009-02-23 | 2010-09-01 | Bayer CropScience AG | Method for improved utilisation of the production potential of genetically modified plants |
US9012360B2 (en) | 2009-03-25 | 2015-04-21 | Bayer Intellectual Property Gmbh | Synergistic combinations of active ingredients |
US8846568B2 (en) | 2009-03-25 | 2014-09-30 | Bayer Cropscience Ag | Active compound combinations having insecticidal and acaricidal properties |
US8846567B2 (en) | 2009-03-25 | 2014-09-30 | Bayer Cropscience Ag | Active compound combinations having insecticidal and acaricidal properties |
EP2232995A1 (en) | 2009-03-25 | 2010-09-29 | Bayer CropScience AG | Method for improved utilisation of the production potential of transgenic plants |
US8828907B2 (en) | 2009-03-25 | 2014-09-09 | Bayer Cropscience Ag | Active ingredient combinations having insecticidal and acaricidal properties |
US8828906B2 (en) | 2009-03-25 | 2014-09-09 | Bayer Cropscience Ag | Active compound combinations having insecticidal and acaricidal properties |
EP2239331A1 (en) | 2009-04-07 | 2010-10-13 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants |
US8835657B2 (en) | 2009-05-06 | 2014-09-16 | Bayer Cropscience Ag | Cyclopentanedione compounds and their use as insecticides, acaricides and/or fungicides |
EP2251331A1 (en) | 2009-05-15 | 2010-11-17 | Bayer CropScience AG | Fungicide pyrazole carboxamides derivatives |
EP3000809A1 (en) | 2009-05-15 | 2016-03-30 | Bayer Intellectual Property GmbH | Fungicide pyrazole carboxamides derivatives |
EP2255626A1 (en) | 2009-05-27 | 2010-12-01 | Bayer CropScience AG | Use of succinate dehydrogenase inhibitors to increase resistance of plants or parts of plants to abiotic stress |
US9232794B2 (en) | 2009-06-02 | 2016-01-12 | Bayer Intellectual Property Gmbh | Use of succinate dehydrogenase inhibitors for controlling Sclerotinia ssp |
US9877482B2 (en) | 2009-06-02 | 2018-01-30 | Bayer Intellectual Property Gmbh | Use of succinate dehydrogenase inhibitors for controlling Sclerotinia ssp |
WO2011006603A2 (en) | 2009-07-16 | 2011-01-20 | Bayer Cropscience Ag | Synergistic active substance combinations containing phenyl triazoles |
WO2011015524A2 (en) | 2009-08-03 | 2011-02-10 | Bayer Cropscience Ag | Fungicide heterocycles derivatives |
EP2292094A1 (en) | 2009-09-02 | 2011-03-09 | Bayer CropScience AG | Active compound combinations |
WO2011035834A1 (en) | 2009-09-02 | 2011-03-31 | Bayer Cropscience Ag | Active compound combinations |
EP2343280A1 (en) | 2009-12-10 | 2011-07-13 | Bayer CropScience AG | Fungicide quinoline derivatives |
WO2011080256A1 (en) | 2009-12-28 | 2011-07-07 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
WO2011080255A2 (en) | 2009-12-28 | 2011-07-07 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
WO2011080254A2 (en) | 2009-12-28 | 2011-07-07 | Bayer Cropscience Ag | Fungicide hydroximoyl-heterocycles derivatives |
WO2011089071A2 (en) | 2010-01-22 | 2011-07-28 | Bayer Cropscience Ag | Acaricide and/or insecticide active substance combinations |
US8722072B2 (en) | 2010-01-22 | 2014-05-13 | Bayer Intellectual Property Gmbh | Acaricidal and/or insecticidal active ingredient combinations |
WO2011107504A1 (en) | 2010-03-04 | 2011-09-09 | Bayer Cropscience Ag | Fluoroalkyl-substituted 2-amidobenzimidazoles and the use thereof for boosting stress tolerance in plants |
WO2011124554A2 (en) | 2010-04-06 | 2011-10-13 | Bayer Cropscience Ag | Use of 4-phenylbutyric acid and/or the salts thereof for enhancing the stress tolerance of plants |
WO2011124553A2 (en) | 2010-04-09 | 2011-10-13 | Bayer Cropscience Ag | Use of derivatives of the (1-cyanocyclopropyl)phenylphosphinic acid, the esters thereof and/or the salts thereof for enhancing the tolerance of plants to abiotic stress |
WO2011134913A1 (en) | 2010-04-28 | 2011-11-03 | Bayer Cropscience Ag | Fungicide hydroximoyl-heterocycles derivatives |
WO2011134911A2 (en) | 2010-04-28 | 2011-11-03 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
WO2011134912A1 (en) | 2010-04-28 | 2011-11-03 | Bayer Cropscience Ag | Fungicide hydroximoyl-heterocycles derivatives |
WO2011151370A1 (en) | 2010-06-03 | 2011-12-08 | Bayer Cropscience Ag | N-[(het)arylalkyl)] pyrazole (thio)carboxamides and their heterosubstituted analogues |
WO2011151368A2 (en) | 2010-06-03 | 2011-12-08 | Bayer Cropscience Ag | Fungicide n-[(trisubstitutedsilyl)methyl]-carboxamide derivatives |
WO2011151369A1 (en) | 2010-06-03 | 2011-12-08 | Bayer Cropscience Ag | N-[(het)arylethyl)] pyrazole(thio)carboxamides and their heterosubstituted analogues |
WO2011154159A1 (en) | 2010-06-09 | 2011-12-15 | Bayer Bioscience N.V. | Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering |
WO2011154158A1 (en) | 2010-06-09 | 2011-12-15 | Bayer Bioscience N.V. | Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering |
WO2012010579A2 (en) | 2010-07-20 | 2012-01-26 | Bayer Cropscience Ag | Benzocycloalkenes as antifungal agents |
WO2012028578A1 (en) | 2010-09-03 | 2012-03-08 | Bayer Cropscience Ag | Substituted fused pyrimidinones and dihydropyrimidinones |
WO2012038480A2 (en) | 2010-09-22 | 2012-03-29 | Bayer Cropscience Ag | Use of biological or chemical control agents for controlling insects and nematodes in resistant crops |
WO2012038476A1 (en) | 2010-09-22 | 2012-03-29 | Bayer Cropscience Ag | Use of active ingredients for controlling nematodes in nematode-resistant crops |
WO2012045798A1 (en) | 2010-10-07 | 2012-04-12 | Bayer Cropscience Ag | Fungicide composition comprising a tetrazolyloxime derivative and a thiazolylpiperidine derivative |
WO2012052489A1 (en) | 2010-10-21 | 2012-04-26 | Bayer Cropscience Ag | 1-(heterocyclic carbonyl) piperidines |
WO2012052490A1 (en) | 2010-10-21 | 2012-04-26 | Bayer Cropscience Ag | N-benzyl heterocyclic carboxamides |
WO2012059497A1 (en) | 2010-11-02 | 2012-05-10 | Bayer Cropscience Ag | N-hetarylmethyl pyrazolylcarboxamides |
WO2012065947A1 (en) | 2010-11-15 | 2012-05-24 | Bayer Cropscience Ag | 5-halogenopyrazolecarboxamides |
WO2012065944A1 (en) | 2010-11-15 | 2012-05-24 | Bayer Cropscience Ag | N-aryl pyrazole(thio)carboxamides |
US9206137B2 (en) | 2010-11-15 | 2015-12-08 | Bayer Intellectual Property Gmbh | N-Aryl pyrazole(thio)carboxamides |
WO2012065945A1 (en) | 2010-11-15 | 2012-05-24 | Bayer Cropscience Ag | 5-halogenopyrazole(thio)carboxamides |
WO2012072696A1 (en) | 2010-12-01 | 2012-06-07 | Bayer Cropscience Ag | Active ingredient combinations comprising pyridylethylbenzamides and other active ingredients |
EP3103339A1 (en) | 2010-12-01 | 2016-12-14 | Bayer Intellectual Property GmbH | Agent combinations comprising pyridylethyl benzamides and other agents |
EP3103338A1 (en) | 2010-12-01 | 2016-12-14 | Bayer Intellectual Property GmbH | Agent combinations comprising pyridylethyl benzamides and other agents |
EP3103340A1 (en) | 2010-12-01 | 2016-12-14 | Bayer Intellectual Property GmbH | Agent combinations comprising pyridylethyl benzamides and other agents |
EP3103334A1 (en) | 2010-12-01 | 2016-12-14 | Bayer Intellectual Property GmbH | Agent combinations comprising pyridylethyl benzamides and other agents |
EP3092900A1 (en) | 2010-12-01 | 2016-11-16 | Bayer Intellectual Property GmbH | Active ingredient combinations comprising pyridylethylbenzamides and other active ingredients |
WO2012072660A1 (en) | 2010-12-01 | 2012-06-07 | Bayer Cropscience Ag | Use of fluopyram for controlling nematodes in crops and for increasing yield |
EP2460406A1 (en) | 2010-12-01 | 2012-06-06 | Bayer CropScience AG | Use of fluopyram for controlling nematodes in nematode resistant crops |
EP2460407A1 (en) | 2010-12-01 | 2012-06-06 | Bayer CropScience AG | Agent combinations comprising pyridylethyl benzamides and other agents |
EP2474542A1 (en) | 2010-12-29 | 2012-07-11 | Bayer CropScience AG | Fungicide hydroximoyl-tetrazole derivatives |
WO2012089757A1 (en) | 2010-12-29 | 2012-07-05 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
WO2012089722A2 (en) | 2010-12-30 | 2012-07-05 | Bayer Cropscience Ag | Use of open-chain carboxylic acids, carbonic esters, carboxamides and carbonitriles of aryl, heteroaryl and benzylsulfonamide or the salts thereof for improving the stress tolerance in plants |
WO2012089721A1 (en) | 2010-12-30 | 2012-07-05 | Bayer Cropscience Ag | Use of substituted spirocyclic sulfonamidocarboxylic acids, carboxylic esters thereof, carboxamides thereof and carbonitriles thereof or salts thereof for enhancement of stress tolerance in plants |
EP2494867A1 (en) | 2011-03-01 | 2012-09-05 | Bayer CropScience AG | Halogen-substituted compounds in combination with fungicides |
WO2012120105A1 (en) | 2011-03-10 | 2012-09-13 | Bayer Cropscience Ag | Use of lipochito-oligosaccharide compounds for safeguarding seed safety of treated seeds |
WO2012123434A1 (en) | 2011-03-14 | 2012-09-20 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
WO2012136581A1 (en) | 2011-04-08 | 2012-10-11 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
EP2511255A1 (en) | 2011-04-15 | 2012-10-17 | Bayer CropScience AG | Substituted prop-2-in-1-ol and prop-2-en-1-ol derivatives |
WO2012139891A1 (en) | 2011-04-15 | 2012-10-18 | Bayer Cropscience Ag | Substituted vinyl and alkinyl cyclohexenols as active agents against abiotic stress in plants |
WO2012139892A1 (en) | 2011-04-15 | 2012-10-18 | Bayer Cropscience Ag | Substituted 5-(bicyclo[4.1.0]hept-3-en-2-yl)-penta-2,4-dienes and 5-(bicyclo[4.1.0]hept-3-en-2-yl)-pent-2-ene-4-ines as active agents against abiotic stress in plants |
WO2012139890A1 (en) | 2011-04-15 | 2012-10-18 | Bayer Cropscience Ag | Substituted 5-(cyclohex-2-en-1-yl)-penta-2,4-dienes and 5-(cyclohex-2-en-1-yl)-pent-2-en-4-ines as active agents against abiotic stress in plants |
EP2997825A1 (en) | 2011-04-22 | 2016-03-23 | Bayer Intellectual Property GmbH | Active compound combinations comprising a (thio)carboxamide derivative and a fungicidal compound |
WO2012168124A1 (en) | 2011-06-06 | 2012-12-13 | Bayer Cropscience Nv | Methods and means to modify a plant genome at a preselected site |
WO2013004652A1 (en) | 2011-07-04 | 2013-01-10 | Bayer Intellectual Property Gmbh | Use of substituted isoquinolinones, isoquinolindiones, isoquinolintriones and dihydroisoquinolinones or in each case salts thereof as active agents against abiotic stress in plants |
WO2013020985A1 (en) | 2011-08-10 | 2013-02-14 | Bayer Intellectual Property Gmbh | Active compound combinations comprising specific tetramic acid derivatives |
US9265252B2 (en) | 2011-08-10 | 2016-02-23 | Bayer Intellectual Property Gmbh | Active compound combinations comprising specific tetramic acid derivatives |
WO2013026836A1 (en) | 2011-08-22 | 2013-02-28 | Bayer Intellectual Property Gmbh | Fungicide hydroximoyl-tetrazole derivatives |
US10538774B2 (en) | 2011-08-22 | 2020-01-21 | Basf Agricultural Solutions Seed, Us Llc | Methods and means to modify a plant genome |
WO2013026740A2 (en) | 2011-08-22 | 2013-02-28 | Bayer Cropscience Nv | Methods and means to modify a plant genome |
US9670496B2 (en) | 2011-08-22 | 2017-06-06 | Bayer Cropscience N.V. | Methods and means to modify a plant genome |
EP2561759A1 (en) | 2011-08-26 | 2013-02-27 | Bayer Cropscience AG | Fluoroalkyl-substituted 2-amidobenzimidazoles and their effect on plant growth |
WO2013034621A1 (en) | 2011-09-09 | 2013-03-14 | Bayer Intellectual Property Gmbh | Acyl-homoserine lactone derivatives for improving plant yield |
WO2013037717A1 (en) | 2011-09-12 | 2013-03-21 | Bayer Intellectual Property Gmbh | Fungicidal 4-substituted-3-{phenyl[(heterocyclylmethoxy)imino]methyl}-1,2,4-oxadizol-5(4h)-one derivatives |
WO2013037955A1 (en) | 2011-09-16 | 2013-03-21 | Bayer Intellectual Property Gmbh | Use of acylsulfonamides for improving plant yield |
WO2013037958A1 (en) | 2011-09-16 | 2013-03-21 | Bayer Intellectual Property Gmbh | Use of phenylpyrazolin-3-carboxylates for improving plant yield |
WO2013037956A1 (en) | 2011-09-16 | 2013-03-21 | Bayer Intellectual Property Gmbh | Use of 5-phenyl- or 5-benzyl-2 isoxazoline-3 carboxylates for improving plant yield |
WO2013041602A1 (en) | 2011-09-23 | 2013-03-28 | Bayer Intellectual Property Gmbh | Use of 4-substituted 1-phenyl-pyrazole-3-carboxylic-acid derivatives as agents against abiotic plant stress |
WO2013050410A1 (en) | 2011-10-04 | 2013-04-11 | Bayer Intellectual Property Gmbh | RNAi FOR THE CONTROL OF FUNGI AND OOMYCETES BY INHIBITING SACCHAROPINE DEHYDROGENASE GENE |
WO2013050324A1 (en) | 2011-10-06 | 2013-04-11 | Bayer Intellectual Property Gmbh | Combination, containing 4-phenylbutyric acid (4-pba) or a salt thereof (component (a)) and one or more selected additional agronomically active compounds (component(s) (b)), that reduces abiotic plant stress |
WO2013075817A1 (en) | 2011-11-21 | 2013-05-30 | Bayer Intellectual Property Gmbh | Fungicide n-[(trisubstitutedsilyl)methyl]-carboxamide derivatives |
WO2013079566A2 (en) | 2011-11-30 | 2013-06-06 | Bayer Intellectual Property Gmbh | Fungicidal n-bicycloalkyl and n-tricycloalkyl (thio)carboxamide derivatives |
WO2013092519A1 (en) | 2011-12-19 | 2013-06-27 | Bayer Cropscience Ag | Use of anthranilic acid diamide derivatives for pest control in transgenic crops |
WO2013098147A1 (en) | 2011-12-29 | 2013-07-04 | Bayer Intellectual Property Gmbh | Fungicidal 3-[(pyridin-2-ylmethoxyimino)(phenyl)methyl]-2-substituted-1,2,4-oxadiazol-5(2h)-one derivatives |
WO2013098146A1 (en) | 2011-12-29 | 2013-07-04 | Bayer Intellectual Property Gmbh | Fungicidal 3-[(1,3-thiazol-4-ylmethoxyimino)(phenyl)methyl]-2-substituted-1,2,4-oxadiazol-5(2h)-one derivatives |
WO2013124275A1 (en) | 2012-02-22 | 2013-08-29 | Bayer Cropscience Ag | Use of succinate dehydrogenase inhibitors (sdhis) for controlling wood diseases in grape. |
WO2013127704A1 (en) | 2012-02-27 | 2013-09-06 | Bayer Intellectual Property Gmbh | Active compound combinations containing a thiazoylisoxazoline and a fungicide |
WO2013139949A1 (en) | 2012-03-23 | 2013-09-26 | Bayer Intellectual Property Gmbh | Compositions comprising a strigolactame compound for enhanced plant growth and yield |
WO2013153143A1 (en) | 2012-04-12 | 2013-10-17 | Bayer Cropscience Ag | N-acyl- 2 - (cyclo) alkylpyrrolidines and piperidines useful as fungicides |
WO2013156559A1 (en) | 2012-04-20 | 2013-10-24 | Bayer Cropscience Ag | N-cycloalkyl-n-[(heterocyclylphenyl)methylene]-(thio)carboxamide derivatives |
WO2013156560A1 (en) | 2012-04-20 | 2013-10-24 | Bayer Cropscience Ag | N-cycloalkyl-n-[(trisubstitutedsilylphenyl)methylene]-(thio)carboxamide derivatives |
WO2013160230A1 (en) | 2012-04-23 | 2013-10-31 | Bayer Cropscience Nv | Targeted genome engineering in plants |
EP2662361A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazol indanyl carboxamides |
EP2662364A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazole tetrahydronaphthyl carboxamides |
EP2662362A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazole indanyl carboxamides |
EP2662363A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole biphenylcarboxamides |
EP2662370A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole benzofuranyl carboxamides |
EP2662360A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole indanyl carboxamides |
WO2013167544A1 (en) | 2012-05-09 | 2013-11-14 | Bayer Cropscience Ag | 5-halogenopyrazole indanyl carboxamides |
WO2013167545A1 (en) | 2012-05-09 | 2013-11-14 | Bayer Cropscience Ag | Pyrazole indanyl carboxamides |
WO2013174836A1 (en) | 2012-05-22 | 2013-11-28 | Bayer Cropscience Ag | Active compounds combinations comprising a lipo-chitooligosaccharide derivative and a nematicide, insecticidal or fungicidal compound |
WO2014009322A1 (en) | 2012-07-11 | 2014-01-16 | Bayer Cropscience Ag | Use of fungicidal combinations for increasing the tolerance of a plant towards abiotic stress |
WO2014037340A1 (en) | 2012-09-05 | 2014-03-13 | Bayer Cropscience Ag | Use of substituted 2-amidobenzimidazoles, 2-amidobenzoxazoles and 2-amidobenzothiazoles or salts thereof as active substances against abiotic plant stress |
WO2014060518A1 (en) | 2012-10-19 | 2014-04-24 | Bayer Cropscience Ag | Method of plant growth promotion using carboxamide derivatives |
WO2014060520A1 (en) | 2012-10-19 | 2014-04-24 | Bayer Cropscience Ag | Method for treating plants against fungi resistant to fungicides using carboxamide or thiocarboxamide derivatives |
WO2014060519A1 (en) | 2012-10-19 | 2014-04-24 | Bayer Cropscience Ag | Method for enhancing tolerance to abiotic stress in plants using carboxamide or thiocarboxamide derivatives |
WO2014060502A1 (en) | 2012-10-19 | 2014-04-24 | Bayer Cropscience Ag | Active compound combinations comprising carboxamide derivatives |
WO2014079957A1 (en) | 2012-11-23 | 2014-05-30 | Bayer Cropscience Ag | Selective inhibition of ethylene signal transduction |
EP2735231A1 (en) | 2012-11-23 | 2014-05-28 | Bayer CropScience AG | Active compound combinations |
WO2014079789A1 (en) | 2012-11-23 | 2014-05-30 | Bayer Cropscience Ag | Active compound combinations |
WO2014083089A1 (en) | 2012-11-30 | 2014-06-05 | Bayer Cropscience Ag | Ternary fungicidal and pesticidal mixtures |
WO2014083033A1 (en) | 2012-11-30 | 2014-06-05 | Bayer Cropsience Ag | Binary fungicidal or pesticidal mixture |
WO2014083031A2 (en) | 2012-11-30 | 2014-06-05 | Bayer Cropscience Ag | Binary pesticidal and fungicidal mixtures |
WO2014083088A2 (en) | 2012-11-30 | 2014-06-05 | Bayer Cropscience Ag | Binary fungicidal mixtures |
WO2014082950A1 (en) | 2012-11-30 | 2014-06-05 | Bayer Cropscience Ag | Ternary fungicidal mixtures |
EP2740356A1 (en) | 2012-12-05 | 2014-06-11 | Bayer CropScience AG | Substituted (2Z)-5(1-Hydroxycyclohexyl)pent-2-en-4-inic acid derivatives |
WO2014086751A1 (en) | 2012-12-05 | 2014-06-12 | Bayer Cropscience Ag | Use of substituted 1-(aryl ethynyl)-, 1-(heteroaryl ethynyl)-, 1-(heterocyclyl ethynyl)- and 1-(cyloalkenyl ethynyl)-cyclohexanols as active agents against abiotic plant stress |
EP2740720A1 (en) | 2012-12-05 | 2014-06-11 | Bayer CropScience AG | Substituted bicyclic and tricyclic pent-2-en-4-inic acid derivatives and their use for enhancing the stress tolerance in plants |
WO2014090765A1 (en) | 2012-12-12 | 2014-06-19 | Bayer Cropscience Ag | Use of 1-[2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfinyl)phenyl]-5-amino-3-trifluoromethyl)-1 h-1,2,4 tfia zole for controlling nematodes in nematode-resistant crops |
WO2014095826A1 (en) | 2012-12-18 | 2014-06-26 | Bayer Cropscience Ag | Binary fungicidal and bactericidal combinations |
WO2014095677A1 (en) | 2012-12-19 | 2014-06-26 | Bayer Cropscience Ag | Difluoromethyl-nicotinic- tetrahydronaphtyl carboxamides |
WO2014135608A1 (en) | 2013-03-07 | 2014-09-12 | Bayer Cropscience Ag | Fungicidal 3-{phenyl[(heterocyclylmethoxy)imino]methyl}-heterocycle derivatives |
WO2014161821A1 (en) | 2013-04-02 | 2014-10-09 | Bayer Cropscience Nv | Targeted genome engineering in eukaryotes |
WO2014167008A1 (en) | 2013-04-12 | 2014-10-16 | Bayer Cropscience Ag | Novel triazolinthione derivatives |
WO2014167009A1 (en) | 2013-04-12 | 2014-10-16 | Bayer Cropscience Ag | Novel triazole derivatives |
WO2014170345A2 (en) | 2013-04-19 | 2014-10-23 | Bayer Cropscience Ag | Method for improved utilization of the production potential of transgenic plants |
WO2014170364A1 (en) | 2013-04-19 | 2014-10-23 | Bayer Cropscience Ag | Binary insecticidal or pesticidal mixture |
WO2014177514A1 (en) | 2013-04-30 | 2014-11-06 | Bayer Cropscience Ag | Nematicidal n-substituted phenethylcarboxamides |
WO2014177582A1 (en) | 2013-04-30 | 2014-11-06 | Bayer Cropscience Ag | N-(2-fluoro-2-phenethyl)carboxamides as nematicides and endoparasiticides |
WO2014206953A1 (en) | 2013-06-26 | 2014-12-31 | Bayer Cropscience Ag | N-cycloalkyl-n-[(bicyclylphenyl)methylene]-(thio)carboxamide derivatives |
WO2015004040A1 (en) | 2013-07-09 | 2015-01-15 | Bayer Cropscience Ag | Use of selected pyridone carboxamides or salts thereof as active substances against abiotic plant stress |
WO2015082587A1 (en) | 2013-12-05 | 2015-06-11 | Bayer Cropscience Ag | N-cycloalkyl-n-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives |
WO2015082586A1 (en) | 2013-12-05 | 2015-06-11 | Bayer Cropscience Ag | N-cycloalkyl-n-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives |
WO2016012362A1 (en) | 2014-07-22 | 2016-01-28 | Bayer Cropscience Aktiengesellschaft | Substituted cyano cycloalkyl penta-2,4-dienes, cyano cycloalkyl pent-2-en-4-ynes, cyano heterocyclyl penta-2,4-dienes and cyano heterocyclyl pent-2-en-4-ynes as active substances against abiotic plant stress |
WO2016096942A1 (en) | 2014-12-18 | 2016-06-23 | Bayer Cropscience Aktiengesellschaft | Use of selected pyridone carboxamides or salts thereof as active substances against abiotic plant stress |
WO2016166077A1 (en) | 2015-04-13 | 2016-10-20 | Bayer Cropscience Aktiengesellschaft | N-cycloalkyl-n-(biheterocyclyethylene)-(thio)carboxamide derivatives |
US11180751B2 (en) | 2015-06-18 | 2021-11-23 | The Broad Institute, Inc. | CRISPR enzymes and systems |
WO2018019676A1 (en) | 2016-07-29 | 2018-02-01 | Bayer Cropscience Aktiengesellschaft | Active compound combinations and methods to protect the propagation material of plants |
WO2018054832A1 (en) | 2016-09-22 | 2018-03-29 | Bayer Cropscience Aktiengesellschaft | Novel triazole derivatives |
WO2018054829A1 (en) | 2016-09-22 | 2018-03-29 | Bayer Cropscience Aktiengesellschaft | Novel triazole derivatives and their use as fungicides |
WO2018054911A1 (en) | 2016-09-23 | 2018-03-29 | Bayer Cropscience Nv | Targeted genome optimization in plants |
WO2018077711A2 (en) | 2016-10-26 | 2018-05-03 | Bayer Cropscience Aktiengesellschaft | Use of pyraziflumid for controlling sclerotinia spp in seed treatment applications |
WO2018104392A1 (en) | 2016-12-08 | 2018-06-14 | Bayer Cropscience Aktiengesellschaft | Use of insecticides for controlling wireworms |
EP3332645A1 (en) | 2016-12-12 | 2018-06-13 | Bayer Cropscience AG | Use of substituted pyrimidine diones or their salts as agents to combat abiotic plant stress |
WO2018108627A1 (en) | 2016-12-12 | 2018-06-21 | Bayer Cropscience Aktiengesellschaft | Use of substituted indolinylmethyl sulfonamides, or the salts thereof for increasing the stress tolerance of plants |
US11591601B2 (en) | 2017-05-05 | 2023-02-28 | The Broad Institute, Inc. | Methods for identification and modification of lncRNA associated with target genotypes and phenotypes |
WO2019025153A1 (en) | 2017-07-31 | 2019-02-07 | Bayer Cropscience Aktiengesellschaft | Use of substituted n-sulfonyl-n'-aryl diaminoalkanes and n-sulfonyl-n'-heteroaryl diaminoalkanes or salts thereof for increasing the stress tolerance in plants |
WO2019060746A1 (en) | 2017-09-21 | 2019-03-28 | The Broad Institute, Inc. | Systems, methods, and compositions for targeted nucleic acid editing |
US10968257B2 (en) | 2018-04-03 | 2021-04-06 | The Broad Institute, Inc. | Target recognition motifs and uses thereof |
WO2019233863A1 (en) | 2018-06-04 | 2019-12-12 | Bayer Aktiengesellschaft | Herbicidally active bicyclic benzoylpyrazoles |
WO2020131862A1 (en) | 2018-12-17 | 2020-06-25 | The Broad Institute, Inc. | Crispr-associated transposase systems and methods of use thereof |
Also Published As
Publication number | Publication date |
---|---|
WO1999012950A3 (en) | 1999-05-06 |
WO1999012950A9 (en) | 1999-06-10 |
EP1009751A2 (en) | 2000-06-21 |
GB9718863D0 (en) | 1997-11-12 |
AU8991198A (en) | 1999-03-29 |
CA2302639A1 (en) | 1999-03-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1009751A2 (en) | Improvements in or relating to stability of plant starches | |
US6956148B1 (en) | Nucleic acids from cassava encoding starch branching enzyme II (SBEII) and their use | |
Woolley et al. | Purification and properties of an endo-β-1, 4-glucanase from strawberry and down-regulation of the corresponding gene, cel1 | |
AU724942B2 (en) | Transgenic potatoes having reduced levels of alpha glucan L- or H-type tuber phosphorylase activity with reduced cold-sweetening | |
US6784338B1 (en) | Genetically engineered modification of potato to form amylopectin-type starch | |
US6570066B1 (en) | Nucleotide sequences encoding enzymes that alter the carbohydrate concentration and composition in plants | |
EP0719338B1 (en) | Combination of dna sequences which enable the formation of modified starch in plant cells and plants, processes for the production of these plants | |
CA2303407C (en) | Regulation of gene expression in plants | |
US5750876A (en) | Isoamylase gene, compositions containing it, and methods of using isoamylases | |
Wang et al. | The essential role of GhPEL gene, encoding a pectate lyase, in cell wall loosening by depolymerization of the de-esterified pectin during fiber elongation in cotton | |
JP2002518015A (en) | Improvement of or related to plants and plant products | |
EP0563201A1 (en) | Genetically engineered modification of potato to form amylose-type starch | |
CA2136828A1 (en) | Dna sequences and plasmids for the preparation of plants with changed sucrose concentration | |
US5767364A (en) | Endo-1,4-beta-D-glucanase | |
HUT73468A (en) | Method of improving the quality of stored potatoes | |
US5856467A (en) | Genetically engineered modification of potato to form amylose-type starch | |
US5569829A (en) | Transformed tomato plants | |
CA2529455A1 (en) | Plant limit dextrinase inhibitor | |
AU727294B2 (en) | Regulation of gene expression in plants | |
KR20050018626A (en) | COFFEE PLANT WITH REDUCED α-D-GALACTOSIDASE ACTIVITY |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AU CA US |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE |
|
AK | Designated states |
Kind code of ref document: A3 Designated state(s): AU CA US |
|
AL | Designated countries for regional patents |
Kind code of ref document: A3 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
AK | Designated states |
Kind code of ref document: C2 Designated state(s): AU CA US |
|
AL | Designated countries for regional patents |
Kind code of ref document: C2 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE |
|
COP | Corrected version of pamphlet |
Free format text: PAGES 1-18, SEQUENCE LISTING, ADDED |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1998941593 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 89911/98 Country of ref document: AU |
|
ENP | Entry into the national phase in: |
Ref country code: CA Ref document number: 2302639 Kind code of ref document: A Format of ref document f/p: F Ref document number: 2302639 Country of ref document: CA |
|
WWP | Wipo information: published in national office |
Ref document number: 1998941593 Country of ref document: EP |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 1998941593 Country of ref document: EP |