WO1998053323A3 - A method for de novo peptide sequence determination - Google Patents

A method for de novo peptide sequence determination Download PDF

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Publication number
WO1998053323A3
WO1998053323A3 PCT/GB1998/001486 GB9801486W WO9853323A3 WO 1998053323 A3 WO1998053323 A3 WO 1998053323A3 GB 9801486 W GB9801486 W GB 9801486W WO 9853323 A3 WO9853323 A3 WO 9853323A3
Authority
WO
WIPO (PCT)
Prior art keywords
peptide
unknown
peptide sequence
sequence determination
fragmentation spectrum
Prior art date
Application number
PCT/GB1998/001486
Other languages
French (fr)
Other versions
WO1998053323A2 (en
Inventor
Raj Bhikhu Parekh
Sally Barbara Prime
Nick Sinclair Wedd
Robert Reid Townsend
Original Assignee
Oxford Glycosciences Uk Ltd
Raj Bhikhu Parekh
Sally Barbara Prime
Nick Sinclair Wedd
Robert Reid Townsend
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Glycosciences Uk Ltd, Raj Bhikhu Parekh, Sally Barbara Prime, Nick Sinclair Wedd, Robert Reid Townsend filed Critical Oxford Glycosciences Uk Ltd
Priority to CA002290591A priority Critical patent/CA2290591A1/en
Priority to AU75403/98A priority patent/AU7540398A/en
Priority to JP55013698A priority patent/JP2002505740A/en
Publication of WO1998053323A2 publication Critical patent/WO1998053323A2/en
Publication of WO1998053323A3 publication Critical patent/WO1998053323A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/0027—Methods for using particle spectrometers
    • H01J49/0036—Step by step routines describing the handling of the data generated during a measurement
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/004—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
    • H01J49/0045—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn characterised by the fragmentation or other specific reaction

Abstract

A method for determining the amino acid sequence of an unknown peptide comprising (a) determining a molecular mass and an experimental fragmentation spectrum for the unknown peptide; (b) comparing the experimental fragmentation spectrum of the unknown peptide to theoretical fragmentation spectra calculated for a peptide library composed of all possible linear sequences of amino acids having a total mass that corresponds to the molecular mass of the unknown peptide; and (c) identifying a peptide in the peptide library having a theoretical fragmentation spectrum that matches most closely the fragmentation spectrum of the unknown peptide.
PCT/GB1998/001486 1997-05-22 1998-05-22 A method for de novo peptide sequence determination WO1998053323A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002290591A CA2290591A1 (en) 1997-05-22 1998-05-22 A method for de novo peptide sequence determination
AU75403/98A AU7540398A (en) 1997-05-22 1998-05-22 A method for de novo peptide sequence determination
JP55013698A JP2002505740A (en) 1997-05-22 1998-05-22 New peptide sequencing method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9710582.9A GB9710582D0 (en) 1997-05-22 1997-05-22 A method for de novo peptide sequence determination
GB9710582.9 1997-05-22

Publications (2)

Publication Number Publication Date
WO1998053323A2 WO1998053323A2 (en) 1998-11-26
WO1998053323A3 true WO1998053323A3 (en) 1999-03-11

Family

ID=10812887

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1998/001486 WO1998053323A2 (en) 1997-05-22 1998-05-22 A method for de novo peptide sequence determination

Country Status (8)

Country Link
US (1) US6582965B1 (en)
EP (1) EP0887646B1 (en)
JP (1) JP2002505740A (en)
AU (1) AU7540398A (en)
CA (1) CA2290591A1 (en)
DE (1) DE69812773D1 (en)
GB (2) GB9710582D0 (en)
WO (1) WO1998053323A2 (en)

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JP2008039608A (en) * 2006-08-07 2008-02-21 Hitachi High-Technologies Corp Mass spectrometry system
US8735806B2 (en) * 2006-11-15 2014-05-27 Shimadzu Corporation Mass-analyzing method and mass spectrometer
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WO2008093762A1 (en) * 2007-01-31 2008-08-07 Chugai Seiyaku Kabushiki Kaisha Sensitive determination method for low molecular weight peptide
WO2008104803A2 (en) 2007-02-26 2008-09-04 Oxford Genome Sciences (Uk) Limited Proteins
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US7555393B2 (en) * 2007-06-01 2009-06-30 Thermo Finnigan Llc Evaluating the probability that MS/MS spectral data matches candidate sequence data
US20120282177A1 (en) 2009-11-02 2012-11-08 Christian Rohlff ROR1 as Therapeutic and Diagnostic Target
CA2811470C (en) * 2010-11-08 2018-03-20 Dh Technologies Development Pte. Ltd. Systems and methods for rapidly screening samples by mass spectrometry
WO2014076529A1 (en) * 2012-11-15 2014-05-22 Dh Technologies Development Pte. Ltd. Systems and methods for identifying compounds from ms/ms data without precursor ion information
WO2017114943A1 (en) * 2015-12-30 2017-07-06 Vito Nv Methods for mass spectrometry-based structure determination of biomacromolecules
US20210020270A1 (en) * 2018-03-08 2021-01-21 The Trustees Of Indiana University Constrained de novo sequencing of neo-epitope peptides using tandem mass spectrometry

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Also Published As

Publication number Publication date
GB9710582D0 (en) 1997-07-16
US6582965B1 (en) 2003-06-24
DE69812773D1 (en) 2003-05-08
EP0887646A1 (en) 1998-12-30
EP0887646B1 (en) 2003-04-02
WO1998053323A2 (en) 1998-11-26
AU7540398A (en) 1998-12-11
JP2002505740A (en) 2002-02-19
GB9811196D0 (en) 1998-07-22
GB2325465A (en) 1998-11-25
CA2290591A1 (en) 1998-11-26

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