CN102262066A - Method and kit for detecting monoamine oxidase content - Google Patents
Method and kit for detecting monoamine oxidase content Download PDFInfo
- Publication number
- CN102262066A CN102262066A CN2011101626108A CN201110162610A CN102262066A CN 102262066 A CN102262066 A CN 102262066A CN 2011101626108 A CN2011101626108 A CN 2011101626108A CN 201110162610 A CN201110162610 A CN 201110162610A CN 102262066 A CN102262066 A CN 102262066A
- Authority
- CN
- China
- Prior art keywords
- monoamine oxidase
- kit
- sample
- dehydrogenase
- aminated compounds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to the technical field of medical examination and measurement and discloses a method and a kit for detecting monoamine oxidase. In the method for detecting the monoamine oxidase, in the absence of hydrogen oxide, the falling speed of nicotinamide-adenine dinucleotide (NADH) reacted with ammonia is measured under the action of glutamate dehydrogenase so as to calculate the monoamine oxidase content; therefore, the accuracy of a detection result is improved; and excessive lactic dehydrogenase and a sample to be detected are mixed, endogenous pyruvic acid of the sample is eliminated within a reaction delay period to make the NADH balanced, so that the accuracy of monoamine oxidase detection is improved. The kit has high stability and a long retention period; and by applying the kit, the detection result is high in accuracy, high in precision and wide in a linear range. The kit is wide in an application range and is convenient to popularize and use and can be applied to the measurement of the monoamine oxidase content of blood serum in all types of hospitals, health prevention departments and medical biological scientific research units.
Description
Technical field
The present invention relates to medical test determination techniques field, relate to a kind of method and kit that detects monoamine oxidase content specifically.
Background technology
(monoamine oxidase MAO) is the enzyme of catalysis monoamine oxidative deamination to monoamine oxidase.Be also referred to as and contain the flavine amine oxidase, can make the enzyme of catechu amine neurotransmitter inactivation, can be used as anti-depression drug.The degree of the active high low energy reflection liver fibrosis of S-MAO is the important indicator of diagnosis cirrhosis.The active positive rate that raises of liver cirrhosis patient S-MAO is more than 80%, and mxm. can surpass the twice of control reference value, and the active rising of S-MAO parallels with the process that liver surface tubercle forms.Various hepatitis patients during acute stage S-MAO activity does not increase, but when in fulminant serious hepatitis or the oxyhepatitis hepatonecrosis being arranged, because mitochondria destroys, the S-MAO activity can raise.Activity of monoamine oxidase raises to be also shown in hyperthyroidism, diabetes and merges diseases such as fatty liver, congestive heart failure, acromegalia.
At present, the assay method of S-MAO has a lot, comprises fluorescence method, immunodepression and chemical photometry.Fluorometry be with tryptamines as substrate, generate heteroauxin through MAO catalysis deamination, the latter produces indoleacetaldehyde through the aldehyde dehydrogenase effect, and makes its coenzyme NAD
+Be reduced to NADH, measure fluorescence in the 370nm place with the excitation wavelength of 280nm.Immunodepression be with the antibody purified bag by microwell plate, make solid phase carrier, add the Avidin of sample or standard items, biotinylated anti-MAO antibody, HRP mark in by the micropore of anti-MAO antibody successively to bag, through thorough washing back with substrate TMB colour developing.TMB changes into blueness under the catalysis of peroxidase, and changes into yellow under the effect of acid.The depth of color and the MAO in the sample are proportionate.Under the 450nm wavelength, measure absorbance with microplate reader, calculation sample concentration.The chemistry photometry is to be substrate with colourless benzylamine, generates benzyl aldehyde through the MAO oxidation, generates the aldehyde phenylhydrazone with 2,4 dinitrophenyl hydrazine again, and the latter is brownish red under alkaline environment, and the depth of color and the MAO in the sample are proportionate.
Yet said method complex operation step, detecting instrument and reagent costliness are not suitable as the routine biochemistry Interventions Requested and use clinically.
Along with widespread usage various half, automatic clinical chemistry analyzer, to develop enzymic colorimetric in recent years and measure monoamine oxidase, its reaction principle is:
Then, under the 340nm wavelength, measure the decline rate of reduced coenzyme NADH absorbance per minute, calculate the concentration of monoamine oxidase.The method of this mensuration monoamine oxidase is widely used in, and widespread usage various half, automatic clinical chemistry analyzer is widely used.Yet the said determination method needs to measure through the hydrogen peroxide route concentration of pyruvic acid, and the hydrogen peroxide poor stability causes the testing result accuracy to reduce.
Summary of the invention
In view of this, the object of the invention provides the method and the kit of the high detection monoamine oxidase content of a kind of accuracy.
For realizing purpose of the present invention, the present invention adopts following technical scheme:
A kind of method that detects monoamine oxidase, for in pH8.8～9.0 damping fluids, oxidation reaction takes place under the effect of the monoamine oxidase of aminated compounds in testing sample generate ammonia, the ammonia of generation under the glutamte dehydrogenase effect with α-Tong Wuersuan reaction consumes reduced coenzyme; Under the 340nm wavelength, detect and write down reduced coenzyme absorbance rate of change in the reactant liquor, calculate the content of monoamine oxidase in the testing sample.
Oxidation reaction takes place under the effect of the monoamine oxidase of detection method aminated compounds of the present invention in testing sample generate ammonia, the ammonia that generates under the glutamte dehydrogenase effect with α-Tong Wuersuan reaction consumes reduced coenzyme, generate glutamic acid and oxidized coenzyme.Reaction equation is:
Monoamine oxidase in reduced coenzyme absorbance decline rate and the testing sample is proportionate, and can calculate the content of the monoamine oxidase in the testing sample by 340nm wavelength reactant liquor absorbance rate of change.
Because the speed of the reaction of monoamine oxidase and aminated compounds is linear, the absorbance rate of change that is per minute is constant, therefore can obtain absorbance rate of change in the entire reaction course according to the absorbance rate of change behind 3～5min, thereby the content of the MAO in the calculating testing sample, computing formula is: C
Sample=Δ A
Sample/ minute * K.Wherein, C
SampleBe testing sample concentration; Δ A
Sample/ minute be sample per minute absorbance rate of change; K is theoretical factor.
Detection method of the present invention is without the hydrogen oxide route, but utilizes the decline rate of the reduced coenzyme of glutamte dehydrogenase effect mensuration and ammonia react to calculate the content of monoamine oxidase, improves the accuracy of testing result.
Because sample itself contains pyruvic acid, consumes reduced coenzyme in course of reaction, may impact reduced coenzyme absorbance decline rate, and then influence the accuracy that MAO detects in the testing sample.Therefore detection method of the present invention comprises that also excessive lactic dehydrogenase mixes with testing sample, catalyzing endogenous property pyruvic acid generation reduction reaction.
In the damping fluid of pH8.8～9.0, lactic dehydrogenase enzymatic sample endogenous pyruvic acid generation reduction reaction generates lactic acid, removes the endogenous pyruvic acid in the testing sample, makes reduced coenzyme reach constant level in 3～5min, and reaction equation is:
Endogenous pyruvic acid reaction in lactic dehydrogenase and the sample is eliminated sample endogenous pyruvic acid in the response delay phase, makes reduced coenzyme reach a balance, has improved the accuracy that monoamine oxidase detects.
As preferably, the described lactic dehydrogenase of detection method of the present invention concentration be 3000～5000U/L.
Preferably, the concentration of described aminated compounds is 15～20mmol/L.
Preferably, the concentration of described glutamte dehydrogenase is 1500～3000U/L.
Preferably, the mol ratio of described aminated compounds of detection method of the present invention and α-Tong Wuersuan is 1: 1.0～1: 1.2.
Because enzyme reaction needs the buffer environment of suitable pH scope and the ionic strength that suits, the ionic strength of damping fluid also affects the activity of enzyme, and ionic strength is too high, electrolyte interferases and substrate combination, enzymatic activity will progressively descend, but ionic strength cross low also can inhibitory enzyme activity.The more approaching ionic strength of body fluid of general selection and physiological environment, therefore detection method of the present invention is carried out in the damping fluid of pH8.8～9.0.Damping fluid of the present invention can be one or more compositions in tris-HCI buffer (Tris-HCl), sodium hydrogen phosphate-potassium phosphate buffer, sodium phosphate disodium hydrogen-phosphate sodium dihydrogen buffer solution, potassium dihydrogen phosphate-sodium hydrate buffer solution, citric acid-sodium citrate damping fluid, the sodium hydrogen phosphate-citrate buffer solution.Preferably, described damping fluid is a tris-HCI buffer.
Detection method of the present invention is with the reaction substrate of aminated compounds as monoamine oxidase, detects the monoamine oxidase in the testing sample, and described aminated compounds is for to do the time spent at highly basic (as NaOH), can make dissociate this compound of amine.Preferably, aminated compounds of the present invention is benzylamine, histamine, butylamine base, amylamine or β-phenethyl amine.Benzylamine more preferably.
The present invention also provides the kit of the high detection monoamine oxidase of a kind of accuracy, comprises damping fluid, 15～20mmol/L aminated compounds, 1.0～1.5mmol/L reduced coenzyme, 10～20mmol/L α-Tong Wuersuan and 1500～3000U/L glutamte dehydrogenase of 80～200mmol/L pH8.8～9.0.
Preferably, the kit of detection monoamine oxidase of the present invention also comprises 3000～5000U/L lactic dehydrogenase.
In a specific embodiments, described kit comprises damping fluid, 18mmol/L aminated compounds, 1.0mmol/L reduced coenzyme, 14mmol/L α-Tong Wuersuan, 1500U/L glutamte dehydrogenase and the 3000U/L lactic dehydrogenase of 100mmol/L pH8.8～9.0.
The kit of detection monoamine oxidase provided by the invention can be single agents, also can be double reagent.For example, damping fluid, aminated compounds, reduced coenzyme, α-Tong Wuersuan, glutamte dehydrogenase and lactic dehydrogenase are made single agents; Perhaps, damping fluid, α-Tong Wuersuan, aminated compounds are made first reagent, damping fluid, reduced coenzyme, glutamte dehydrogenase and lactic dehydrogenase are made second reagent, when utilizing this kit to detect monoamine oxidase, with first reagent and second reagent mix, thus the monoamine oxidase in the detection testing sample.
Reagent in the kit of detection monoamine oxidase provided by the invention can be dry powder, dissolves with damping fluid before using; Also being mixed with liquid reagent directly uses.
From above-mentioned technical scheme as can be seen, oxidation reaction takes place under the effect of the monoamine oxidase of method aminated compounds in testing sample of detection monoamine oxidase provided by the invention generate ammonia, the ammonia of generation under the glutamte dehydrogenase effect with α-Tong Wuersuan reaction consumes reduced coenzyme; Under the 340nm wavelength, detect and write down reduced coenzyme absorbance rate of change in the reactant liquor, calculate the content of monoamine oxidase in the testing sample.Detection method of the present invention is without the hydrogen oxide route, but utilizes the decline rate of the reduced coenzyme of glutamte dehydrogenase effect mensuration and ammonia react to calculate the content of monoamine oxidase, improves the accuracy of testing result.Detection method of the present invention comprises that also excessive lactic dehydrogenase mixes with testing sample, catalyzing endogenous property pyruvic acid generation reduction reaction.Endogenous pyruvic acid and reaction in lactic dehydrogenase and the sample are eliminated sample endogenous pyruvic acid in the response delay phase, make reduced coenzyme reach a balance, have improved the accuracy that monoamine oxidase detects.
Kit provided by the invention comprises damping fluid, 15～20mmol/L aminated compounds, 1.0～1.5mmol/L reduced coenzyme, 10～20mmol/L α-Tong Wuersuan and 1500～3000U/L glutamte dehydrogenase of 80～200mmol/L pH8.8～9.0.Kit good stability of the present invention, long shelf-life is used kit testing result accuracy height of the present invention, precision is good, the range of linearity is wide.Kit of the present invention is applied widely, is convenient to promote the use of, and can be applicable to hospitals at different levels, sanitary precaution department and medical biotechnology R﹠D institution and measures monoamine oxidase content in the serum.
Embodiment
The embodiment of the invention discloses a kind of method and kit that detects monoamine oxidase.Those skilled in the art can use for reference this paper content, suitably improve technological parameter and realize.Special needs to be pointed out is that all similarly replace and change apparent to those skilled in the art, they all are regarded as being included in the present invention.Method of the present invention and product are described by preferred embodiment, the related personnel obviously can be in not breaking away from content of the present invention, spirit and scope to method as herein described with product is changed or suitably change and combination, realize and use the technology of the present invention.
In order further to understand the present invention, the present invention is described in detail below in conjunction with embodiment.
Embodiment 1: the kit of detection monoamine oxidase of the present invention
The kit of detection monoamine oxidase of the present invention is the liquid single agents, comprising: the Tris-HCl damping fluid of 100mmol/LpH8.8～9.0,18mmol/L aminated compounds, 1.5mmol/L reduced coenzyme, 14mmol/L α-Tong Wuersuan, 1500U/L glutamte dehydrogenase and 3000U/L lactic dehydrogenase.
Embodiment 2: the kit of detection monoamine oxidase of the present invention
The kit of detection monoamine oxidase of the present invention is a double reagent, first reagent comprises Tris-HCl damping fluid, 10mmol/L α-Tong Wuersuan, the 15mmol/L aminated compounds of 80mmol/LpH9.1～9.2, and second reagent comprises Tris-HCl damping fluid, 1.0mmol/L reduced coenzyme, 2000U/L glutamte dehydrogenase and the 4000U/L lactic dehydrogenase of 50mmol/L pH8.6～8.8.
Embodiment 3: the kit of detection monoamine oxidase of the present invention
The kit of detection monoamine oxidase of the present invention is the liquid single agents, comprising: the Tris-HCl damping fluid of 200mmol/LpH8.8～9.0,20mmol/L aminated compounds, 1.5mmol/L reduced coenzyme, 20mmol/L α-Tong Wuersuan, 3000U/L glutamte dehydrogenase and 5000U/L lactic dehydrogenase.
Embodiment 4: the method that detects monoamine oxidase
Set 37 ℃ of automatic clinical chemistry analyzer temperature of reaction, reaction method is a rate method, measures predominant wavelength 340nm, and the Direction of Reaction is negative reaction, time delay 300s, Measuring Time is 180s, theoretical factor is 1768.Get then in testing sample and the kit reagent mix evenly after, place automatic clinical chemistry analyzer, detect and record 340nm wavelength under the absorbance changing value.According to computing formula C
Sample=Δ A
Sample/ min * K, the concentration of monoamine oxidase in the calculating testing sample, wherein, C
SampleBe sample to be tested concentration; Δ A
Sample/ min is a sample per minute absorbance rate of change; K is theoretical factor, has been set at 1768.
Embodiment 5: detection method accuracy of the present invention detects
With the monoamine oxidase solution of variable concentrations as testing sample, reagent mix with the embodiment of the invention 1 described kit, according to embodiment 4 described methods (detection method of the present invention), kit is tested, utilize the monoamine oxidase content in Hitachi's 7080 automatic clinical chemistry analyzers detection testing sample, each concentration samples replication 3 times, the mean value of calculating sample measurement result
Be calculated as follows relative deviation (B%), the results are shown in Table 1.
Table 1 variable concentrations sample monoamine oxidase testing result
By table 1 result as seen, detection method B% of the present invention＜2% meets " the general requirement of external diagnosis reagent ", shows that detection method accuracy of the present invention is good.
Embodiment 6: kit repeatability of the present invention detects
As testing sample,,, utilize the monoamine oxidase content in Hitachi's 7080 automatic clinical chemistry analyzers detection testing sample with conventional serum sample, the results are shown in Table 2 according to embodiment 4 described methods respectively with embodiment 1 described kit reagent mix.
Table 2 sample repeatability testing result
| Testing sample | Embodiment 1 kit |
| 1 | 54.71 |
| 2 | 56.23 |
| 3 | 54.74 |
| 4 | 54.45 |
| 5 | 53.9 |
| 6 | 56.24 |
| 7 | 54.12 |
| 8 | 54.74 |
| 9 | 56.49 |
| 10 | 55.86 |
| CV％ | 1.74％ |
By the result of table 2 as seen, adopt kit of the present invention to detect the coefficient of variation CV=1.74% of monoamine oxidase,, meet " the general requirement of external diagnosis reagent " less than 5%, show kit good reproducibility of the present invention.
Embodiment 7: the detection of kit linearity of the present invention
Get the high value serum of monoamine oxidase concentration near 100U/L, be diluted to 5 different concentration gradients, the theoretical concentration value is followed successively by 6.25,12.5,25,50,100U/L, mix with embodiment 1 described kit,, utilize the monoamine oxidase in Hitachi's 7080 automatic clinical chemistry analyzers detection testing sample according to embodiment 4 described methods, each concentration determination 2 times, average, calculate the correlation coefficient r value, the results are shown in Table 3 according to formula.
Table 3 sample result of linear detection
By the result of table 3 as seen, the kit range of linearity of the present invention can reach 100U/L, shows that the kit range of linearity of the present invention is wide, applied widely.
The explanation of above embodiment just is used for helping to understand method of the present invention and core concept thereof.Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention, can also carry out some improvement and modification to the present invention, these improvement and modification also fall in the protection domain of claim of the present invention.
Claims (10)
1. method that detects monoamine oxidase content, it is characterized in that, in the damping fluid of pH8.8～9.0, oxidation reaction takes place under the effect of the monoamine oxidase of aminated compounds in testing sample generate ammonia, the ammonia of generation under the glutamte dehydrogenase effect with α-Tong Wuersuan reaction consumes reduced coenzyme; Under the 340nm wavelength, detect and write down reduced coenzyme absorbance rate of change in the reactant liquor, calculate the content of monoamine oxidase in the testing sample.
2. according to the described detection method of claim 1, it is characterized in that, comprise that also excessive lactic dehydrogenase mixes with testing sample, catalyzing endogenous property pyruvic acid generation reduction reaction.
3. according to the described detection method of claim 2, it is characterized in that the concentration of described lactic dehydrogenase is 3000～5000U/L.
4. according to claim 1 or 2 described detection methods, it is characterized in that the concentration of described aminated compounds is 15～20mmol/L.
5. according to claim 1 or 2 described detection methods, it is characterized in that the concentration of described glutamte dehydrogenase is 1500～3000U/L.
6. according to claim 1 or 2 described detection methods, it is characterized in that the mol ratio of described aminated compounds and α-Tong Wuersuan is 1: 1.0～1: 1.2.
7. according to claim 1 or 2 described detection methods, it is characterized in that described damping fluid is a tris-HCI buffer.
8. according to claim 1 or 2 described detection methods, it is characterized in that described aminated compounds is benzylamine, histamine, butylamine base, amylamine or β-phenethyl amine.
9. kit that detects monoamine oxidase content, it is characterized in that, comprise tris-HCI buffer, 15～20mmol/L aminated compounds, 1.0～1.5mmol/L reduced coenzyme, 10～20mmol/L α-Tong Wuersuan and 1500～3000U/L glutamte dehydrogenase of 80～200mmol/LpH8.8～9.0.
10. according to the described kit of claim 9, it is characterized in that, also comprise 3000～5000U/L lactic dehydrogenase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101626108A CN102262066A (en) | 2011-06-16 | 2011-06-16 | Method and kit for detecting monoamine oxidase content |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101626108A CN102262066A (en) | 2011-06-16 | 2011-06-16 | Method and kit for detecting monoamine oxidase content |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102262066A true CN102262066A (en) | 2011-11-30 |
Family
ID=45008777
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101626108A Pending CN102262066A (en) | 2011-06-16 | 2011-06-16 | Method and kit for detecting monoamine oxidase content |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102262066A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861628A (en) * | 2016-04-28 | 2016-08-17 | 安徽伊普诺康生物技术股份有限公司 | Kit for measuring monoamine oxidase and preparation method thereof |
| CN106770716A (en) * | 2016-11-28 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | Histamine detection method and kit in a kind of animal tissue |
| CN108196075A (en) * | 2017-12-27 | 2018-06-22 | 山东博科生物产业有限公司 | A kind of detection reagent and detection method of apotryptophanase method detection blood potassium ion |
| CN116497084A (en) * | 2023-06-09 | 2023-07-28 | 中拓生物有限公司 | Anti-interference stable serum monoamine oxidase assay kit and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999029688A1 (en) * | 1997-12-05 | 1999-06-17 | Pharmacia & Upjohn Company | S-oxide and s,s-dioxide tetrahydrothiopyran phenyloxazolidinones |
| CN1789428A (en) * | 2004-12-13 | 2006-06-21 | 苏州艾杰生物科技有限公司 | Monoamine oxidase activity determination method and monoamine oxidase diagnostic kit |
| CN101498662A (en) * | 2008-01-29 | 2009-08-05 | 北京九强生物技术有限公司 | Reagent kit for monoamine oxidase MAO single-reagent measurement |
-
2011
- 2011-06-16 CN CN2011101626108A patent/CN102262066A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999029688A1 (en) * | 1997-12-05 | 1999-06-17 | Pharmacia & Upjohn Company | S-oxide and s,s-dioxide tetrahydrothiopyran phenyloxazolidinones |
| CN1789428A (en) * | 2004-12-13 | 2006-06-21 | 苏州艾杰生物科技有限公司 | Monoamine oxidase activity determination method and monoamine oxidase diagnostic kit |
| CN101498662A (en) * | 2008-01-29 | 2009-08-05 | 北京九强生物技术有限公司 | Reagent kit for monoamine oxidase MAO single-reagent measurement |
Non-Patent Citations (2)
| Title |
|---|
| 倪星忠: "《临床生化酶试剂方法》", 31 March 1993, article "柠檬酸的测定", pages: 221-223 * |
| 科斯蒂尔 等: "《运动医学与科学手册 游泳》", 31 July 2002, article "附录" * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861628A (en) * | 2016-04-28 | 2016-08-17 | 安徽伊普诺康生物技术股份有限公司 | Kit for measuring monoamine oxidase and preparation method thereof |
| CN106770716A (en) * | 2016-11-28 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | Histamine detection method and kit in a kind of animal tissue |
| CN108196075A (en) * | 2017-12-27 | 2018-06-22 | 山东博科生物产业有限公司 | A kind of detection reagent and detection method of apotryptophanase method detection blood potassium ion |
| CN116497084A (en) * | 2023-06-09 | 2023-07-28 | 中拓生物有限公司 | Anti-interference stable serum monoamine oxidase assay kit and preparation method and application thereof |
| CN116497084B (en) * | 2023-06-09 | 2024-03-29 | 中拓生物有限公司 | Anti-interference stable serum monoamine oxidase assay kit and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100595282C (en) | Glutamate-pyruvate transaminase determination reagent kit | |
| CN104198408A (en) | Detection kit for determining content of creatinine in serum by enzymic method | |
| CN102175670A (en) | Method for detecting 1,5-dehydration glucitol in blood and kit | |
| CN107505273A (en) | Serum tolal bile acid assay kit and its application method | |
| CN102262066A (en) | Method and kit for detecting monoamine oxidase content | |
| CN103048282B (en) | Detection method of bilirubin and detection kit | |
| CN102253041B (en) | Creatinine detection kit | |
| CN101498662A (en) | Reagent kit for monoamine oxidase MAO single-reagent measurement | |
| CN106086158A (en) | A kind of test kit measuring α HBD and preparation method thereof | |
| CN102747133B (en) | Adenosine deaminase (ADA) detection reagent kit and preparation method thereof | |
| CN104049091B (en) | A kind of method and test kit detecting ethanol dehydrogenase | |
| CN110082306A (en) | Monoamine oxidase assay kit | |
| CN103088108A (en) | Kit for detecting glucose by using glucose dehydrogenase method and preparation method | |
| CN102081082A (en) | Determination method of galactose and galactose diagnosis/determination kit | |
| CN102297943A (en) | Galactose measurement method and galactose diagnosis/measurement kit | |
| CN102081098A (en) | Galactose determination method and galactose diagnosis/assay kit | |
| CN102297979A (en) | Measuring method and diagnosing/measuring reagent kit for galactose | |
| CN102297980A (en) | Measuring method and diagnosing/measuring reagent kit for galactose | |
| CN102297975A (en) | Measuring method and diagnosing/measuring reagent kit for galactose | |
| CN102297976A (en) | Method for determining galactose and galactose diagnosis/determination kit | |
| CN102081086A (en) | Measurement method and diagnosis/measurement kit of galactose | |
| CN102081087A (en) | Galactose determination method and galactose diagnosis/assay kit | |
| CN102081084A (en) | Method for assaying galactose and galactose diagnosis/assay kit | |
| CN102297946A (en) | Method for determining galactose and galactose diagnosis/determination kit | |
| CN102081081A (en) | Measurement method and diagnosis/measurement kit of galactose |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20111130 |