CA2400993A1 - Carriers coated with polysaccharides, their preparation and use - Google Patents

Carriers coated with polysaccharides, their preparation and use Download PDF

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Publication number
CA2400993A1
CA2400993A1 CA002400993A CA2400993A CA2400993A1 CA 2400993 A1 CA2400993 A1 CA 2400993A1 CA 002400993 A CA002400993 A CA 002400993A CA 2400993 A CA2400993 A CA 2400993A CA 2400993 A1 CA2400993 A1 CA 2400993A1
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CA
Canada
Prior art keywords
carrier
functional groups
polysaccharide
layer
carrier according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002400993A
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French (fr)
Other versions
CA2400993C (en
Inventor
Hrair Kirakossian
John S. Pease
Carsten Schelp
Marcel R. Pirio
Uwe Stohr
Andreas Wiegand
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Siemens Healthcare Diagnostics Products GmbH
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Individual
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Filing date
Publication date
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Publication of CA2400993A1 publication Critical patent/CA2400993A1/en
Application granted granted Critical
Publication of CA2400993C publication Critical patent/CA2400993C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/548—Carbohydrates, e.g. dextran
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/969—Multiple layering of reactants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/827—Lectins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]
    • Y10T428/2991—Coated

Abstract

A polysaccharide coated carrier having a coating of at least two successive layers of polysaccharide. The first polysaccharide layer spontaneously associates with a second polysaccharide layer and, optionally, the carrier. Each successive layer of polysaccharide spontaneously associates with a preceding layer. Spontaneous association occurs due to the presence of oppositely charged functional groups on each layer of polysaccharide or due to a spontaneous reaction between the functional groups the layers. The carrier may be any surface such as a tube, microtitration plate, bead, particle or t he like and is suitable for use in diagnostic or therapeutic methods.

Claims (39)

1. A polysaccharide coated carrier comprising a carrier having a coating of at least two successive layers of polysaccharides, wherein a first polysaccharide layer spontaneously associates with a second polysaccharide layer.
2. The carrier of claim 1 wherein each of said successive polysaccharide layer spontaneously associates with each of a preceding polysaccharide layer.
3. The carrier of claim 1 wherein said polysaccharides have pendent functional groups and said functional groups of said successive polysaccharide layers are charged oppositely of said functional groups of said preceding polysaccharide layers.
4. The carrier of claim 1 wherein said polysaccharides have pendant functional groups and said successive layers of polysaccharide are covalently coupled to said preceding polysaccharide layers by reaction between said functional groups of said successive layers with said functional groups of said preceding layers.
5. The carrier of claim 4 wherein the reaction between said functional groups is a spontaneous reaction.
6. The carrier according to claim 4 wherein said functional groups of said successive polysaccharide layers alternate between amine functional groups and amine-reactive functional groups.
7. The carrier according to claim 6 wherein the amine reactive functional group is an aldehyde group or a carboxyl group.
8. The carrier of claim 1 wherein said first polysaccharide layer spontaneously associates with said carrier.
9. ~The carrier of claim 8 wherein said carrier and said polysaccharides have pendent functional groups
10. The carrier of claim 9 wherein said functional groups of said carrier and said functional groups of said first polysaccharide layer are the cause of the spontaneous association.
11. The carrier of claim 9 wherein said functional groups of said carrier and said functional groups of said first polysaccharide layer are oppositely charged.
12. The carrier of claim 9 wherein said carrier and said polysaccharides have pendent functional groups, and said first polysaccharide layer is covalently coupled to said carrier by reaction between said functional groups of said carrier and said functional groups of said first polysaccharide layer.
13. The carrier of claim 12 wherein the reaction between said functional groups is a spontaneous reaction.
14. The carrier according to claim 12 wherein said functional groups of said carrier is an amine reactive functional groups and the functional groups of said first polysaccharide layer are amine functional groups.
15. The carrier of claim 14 wherein said amine reactive functional group is an aldehyde group or a carboxyl group.
16. The carrier according to any one of claims 1 through 15 wherein said carrier material is selected from the group consisting of natural, synthetic or modified naturally occurring polymers such as agarose, cellulose, nitrocellulose,~
cellulose acetate, polyvinylchloride, polystyrene, polyethylene, polypropylene,~
poly(4-methylbutene), polyacrylamide, polymethacrylate, polyethyleneterephthalate, nylon, polyvinylbutyrate or polyacrylat; silicones;
glasses; ceramics; inorganic powders such as silica, magnesium sulfate and alumina; magnetic materials; metals; or a combination thereof.
17. The carrier according to any one of claims 1 through 16 wherein said carrier is selected from the group consisting of strips, sheets, rods, tubes, wells, microtitration plates, beads and particles.
18. The carrier according to claim 17 wherein said carrier is a magnetic or non-magnetic particle.
19. The carrier according to claim 18 wherein said carrier is a carboxylated latex particle.
20. The carrier according to claim 18 wherein said particle is in the size range of 0.1 to 10 µm, preferably 0.1 to 5 µm, most preferably 0.15 to 3 µm.
21. The carrier according to any one of claims 1 through 20 wherein said carrier is associated with at least one reporter molecule.
22. The carrier according to claim 21 wherein said reporter molecule is selected from the group consisting of dyes, radiolabels, sensitizers, fluorescers and/or chemiluminescers.
23. The carrier according to any one of claims 1 through 22 wherein the polysaccharide of the first coating layer is an aminodextran.
24. The carrier according to claim 23 wherein said aminodextran has a molecular weight of 10,000 to 2,000,000.
25. The carrier according to any of the preceding claims wherein the outermost layer of polysaccharide has at least one pendent functional group.
26. The carrier according to claim 25 wherein said pendent functional group is selected from the group consisting of amines, aldehydes, carboxyl groups, maleimido groups, or sulfhydryl groups.
27. The carrier according to claims 25 or 26 wherein said pendent functional group is bound to a specific binding partner.
28. The carrier according to claim 27 wherein said specific binding partner is selected from the group consisting of antibodies, antibody fragments, receptors, ligands, oligonucleotides, oligonucleotide-binding proteins, lectins, haptens, antigens, immunoglobulin binding proteins, avidin, streptavidin, or biotin.
29. A method for preparing the polysaccharide coated carrier of claim 1 comprising:

(a) covalently coupling the first polysaccharide layer to said carrier by reaction between the amine reactive functional groups of the carrier and the amine functional groups of the polysaccharides of the first coating layer; and (b) covalently coupling the second polysaccharide layer to the first polysaccharide layer by reaction between the amine functional groups of the first polysaccharide layer and the amine reactive functional groups of the second polysaccharide layer.
30. A method for preparing the polysaccharide coated carrier of claim 1 comprising:

(a) covalently coupling the first polysaccharide layer to said carrier by reaction between the amine functional groups of the carrier and the amine reactive functional groups of the polysaccharides of the first coating layer; and (b) covalently coupling the second polysaccharide layer to the first polysaccharide layer by reaction between the amine reactive functional groups of the first polysaccharide layer and the amine functional groups of the second polysaccharide layer.
31. The method according to claims 29 or 30 wherein carbodiimide conjugation chemistry is used for the coupling of the polysaccharides of the first coating layer to said carrier.
32. The method according to any one of claims 29 through 31 wherein the coupling of the polysaccharides of the second coating layer to the first coating layer is done in the presence of a mild reducing agent, such as sodium cyanoborhydride.
33. The method according to any one of claims 29 through 31 wherein carbodiimide conjugation chemistry is used for the coupling of the polysaccharides of the second coating layer to the first coating layer.
34. The method according to any one of claims 29 through 33 wherein the specific binding partners are covalently bound to the coating layer by reaction between the pendent functional groups of the coating layer and functional groups of the specific binding partners.
35. The carrier according to any one of claims 1 through 28 for use in in-vitro and/or in-vivo diagnostic methods.
36. The carrier according to any one of claims 1 through 28 for use in a method for the quantitative and/or qualitative determination of an analyte.
37. Composition comprising the carrier according to any one of claims 1 through 28 in a pharmaceutically acceptable medium.
38. The carrier according to any one of claims 1 through 28 for use in a therapeutic method.
39. A method for quantitative or qualitative determination of an analyte in a sample using the carrier according to any one of claims 1 through 28.
CA2400993A 2000-03-06 2000-03-06 Carriers coated with polysaccharides, their preparation and use Expired - Lifetime CA2400993C (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2000/005978 WO2001067105A1 (en) 2000-03-06 2000-03-06 Carriers coated with polysaccharides, their preparation and use

Publications (2)

Publication Number Publication Date
CA2400993A1 true CA2400993A1 (en) 2001-09-13
CA2400993C CA2400993C (en) 2011-06-07

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Family Applications (1)

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CA2400993A Expired - Lifetime CA2400993C (en) 2000-03-06 2000-03-06 Carriers coated with polysaccharides, their preparation and use

Country Status (7)

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US (1) US7179660B1 (en)
EP (1) EP1264181B1 (en)
JP (1) JP4448642B2 (en)
CA (1) CA2400993C (en)
DE (1) DE60035127T2 (en)
ES (1) ES2287006T3 (en)
WO (1) WO2001067105A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
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CN113125703A (en) * 2019-12-31 2021-07-16 博阳生物科技（上海）有限公司 Homogeneous detection kit for myoglobin and application thereof

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020164825A1 (en) * 2000-09-09 2002-11-07 Wen-Tien Chen Cell separation matrix
EP1546723A4 (en) * 2002-08-16 2007-03-07 Decision Biomarkers Inc Reading of fluorescent arrays
DE10318801A1 (en) * 2003-04-17 2004-11-04 Aesculap Ag & Co. Kg Flat implant and its use in surgery
EP1494028A1 (en) * 2003-07-03 2005-01-05 Labsoft Diagnostics AG Immunomagnetic separation of specific target cells
JP4665161B2 (en) * 2004-02-06 2011-04-06 国立大学法人高知大学 Method for measuring Raman spectrum, noble metal particles used in the method, and method for producing the noble metal particles
CN104090097B (en) * 2004-02-26 2017-04-12 康多尔生物技术有限公司 Aqueous solution of specificity binding interaction culture medium serving as binding pair
US7887880B2 (en) * 2004-06-30 2011-02-15 Auburn University Preparation and application of stabilized iron nanoparticles for dechlorination of chlorinated hydrocarbons in soils, sediments, and ground water
US20060040258A1 (en) * 2004-08-23 2006-02-23 Huiyan Guo Water-soluble conjugates and methods of preparation
DE102004042124B4 (en) * 2004-08-30 2006-07-06 Dade Behring Marburg Gmbh Homogeneous method for the determination of an analyte in the presence of an excess of cross-reacting substances
CA2595292C (en) * 2005-01-20 2014-03-04 Luminex Corporation Magnetic microspheres for use in fluorescence-based applications
US20060166376A1 (en) * 2005-01-21 2006-07-27 Craig Alan R Compositions for use as a signal generation component and methods of using same
EP1715344A1 (en) * 2005-04-22 2006-10-25 Universiteit Utrecht Holding B.V. Immobilisation of antigenic carbohydrates to support detection of pathogenic micro-organisms
JP2006321932A (en) * 2005-05-20 2006-11-30 Jsr Corp Carrier polymer particle and its production method
JP4873123B2 (en) * 2005-11-28 2012-02-08 Ｊｓｒ株式会社 Method for producing carrier polymer particles
WO2006123686A1 (en) * 2005-05-20 2006-11-23 Jsr Corporation Support polymer particle, process for producing the same, magnetic particle for specific trapping, and process for producing the same
JP5007920B2 (en) * 2005-08-31 2012-08-22 日立金属株式会社 Nucleic acid extraction method, cancer cell detection method and magnetic beads
SG131016A1 (en) 2005-09-19 2007-04-26 Millipore Corp Asymmetric porous adsorptive bead
MX2008007716A (en) 2005-12-13 2008-09-26 Exthera Ab Method for extracorporeal removal of a pathogenic microbe, an inflammatory cell or an inflammatory protein from blood.
EP2015071A1 (en) * 2007-07-13 2009-01-14 FUJIFILM Corporation Carrier, process for producing same, bioreactor, and chip for surface plasmon resonance analysis
WO2009064977A2 (en) * 2007-11-14 2009-05-22 E.I. Du Pont De Nemours And Company Dextran-based polymer tissue adhesive for medical use
CA2723076A1 (en) * 2008-05-09 2009-11-12 Zhiping Liu A molecule detecting system
JP5253974B2 (en) * 2008-11-14 2013-07-31 株式会社コーセー MULTILAYER COATED PARTICLE, PROCESS FOR PRODUCING THE SAME, AND EXTERNAL SKIN CONTAINING THE SAME
US20120149128A1 (en) * 2009-02-23 2012-06-14 Victor Manneh Assays and assay devices
EP2230515B1 (en) 2009-03-16 2014-12-17 Agilent Technologies, Inc. Passivation of surfaces after ligand coupling
US20120225496A1 (en) 2009-11-17 2012-09-06 Satoru Yoshida Organic colored microparticles, diagnostic reagent kit containing the same, and in vitro diagnosis method
CN106178161B (en) 2009-12-01 2020-12-22 艾克塞拉医疗公司 Method for removing cytokines from blood using surface immobilized polysaccharides
CN102109521A (en) * 2010-12-16 2011-06-29 中国环境科学研究院 Immunolatex microsphere for detecting CpTI and preparation method thereof
WO2012112724A1 (en) 2011-02-15 2012-08-23 Exthera Medical, Llc Device and method for removal of blood-borne pathogens, toxins and inflammatory cytokines
US8394647B2 (en) 2011-02-17 2013-03-12 Siemens Healthcare Diagnostics Inc. Reducing non-covalently bound polysaccharide on supports
EP2541250A1 (en) * 2011-06-30 2013-01-02 Koninklijke Philips Electronics N.V. Molecular architecture on magnetic particles for affinity assays with low non-specific binding
WO2013070653A1 (en) * 2011-11-09 2013-05-16 Board Of Trustees Michigan State University Metallic nanoparticle synthesis with carbohydrate capping agent
JP6226958B2 (en) * 2012-04-18 2017-11-08 シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッドＳｉｅｍｅｎｓ Ｈｅａｌｔｈｃａｒｅ Ｄｉａｇｎｏｓｔｉｃｓ Ｉｎｃ． COMPOUNDS AND METHODS FOR PRODUCING CONJUGATE REAGENTS
EP2861273B1 (en) 2012-06-13 2017-08-23 ExThera Medical Corporation Use of heparin and carbohydrates to treat cancer
US9244083B2 (en) 2012-11-30 2016-01-26 Siemens Healthcare Diagnostics Inc. Compositions and methods for detecting vitamin D
US20140308751A1 (en) 2013-04-12 2014-10-16 Siemens Healthcare Diagnostics Inc. Assays for Analyte Homologs
EP3013445B1 (en) * 2013-06-24 2019-11-06 ExThera Medical Corporation Blood filtration system containing mannose coated substrate
US10124072B2 (en) * 2013-09-18 2018-11-13 Caliper Life Sciences, Inc. In-vivo reactive species imaging
WO2015069942A1 (en) 2013-11-08 2015-05-14 Exthera Medical Corporation Methods for diagnosing infectious diseases using adsorption media
BR112016024569B1 (en) 2014-04-24 2023-01-03 Exthera Medical Corporation EX VIVO METHOD FOR REMOVING BACTERIA FROM A SPECIMEN TAKEN FROM AN INDIVIDUAL WHO IS SUSPECTED TO BE INFECTED WITH THE SAME
JP6681349B2 (en) 2014-06-27 2020-04-15 シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッドＳｉｅｍｅｎｓ Ｈｅａｌｔｈｃａｒｅ Ｄｉａｇｎｏｓｔｉｃｓ Ｉｎｃ． Binding partners specific for vitamin D epimers
KR20170016009A (en) 2014-06-27 2017-02-10 지멘스 헬쓰케어 다이아그노스틱스 인크. Binding partners specific for vitamin d epimers in vitamin d assays
EP3197342B1 (en) 2014-09-22 2020-08-05 ExThera Medical Corporation Wearable hemoperfusion device
JP6852259B2 (en) * 2016-01-07 2021-03-31 昭和電工マテリアルズ株式会社 Separator and column
US11911551B2 (en) 2016-03-02 2024-02-27 Exthera Medical Corporation Method for treating drug intoxication
EP3422943A4 (en) 2016-03-02 2019-10-16 ExThera Medical Corporation Method for treating drug intoxication
CN116337851A (en) * 2018-07-18 2023-06-27 科美博阳诊断技术(上海)有限公司 Homogeneous chemiluminescence detection kit and application thereof
CN116559151A (en) * 2018-07-18 2023-08-08 上海索昕生物科技有限公司 Microsphere composition for chemiluminescence detection and application thereof
CN110736737A (en) * 2018-07-18 2020-01-31 博阳生物科技（上海）有限公司 microsphere composition for chemiluminescence detection and application thereof
US20210247398A1 (en) 2018-07-30 2021-08-12 Siemens Healthcare Diagnostics Inc. Kits, microfluidics devices, and methods for performing biotin assays
WO2020068541A1 (en) 2018-09-25 2020-04-02 Siemens Healthcare Diagnostics Inc. Methods and compositions for removing biotin interference from assays using molecular traps
CN116626285A (en) * 2019-07-19 2023-08-22 科美博阳诊断技术(上海)有限公司 Homogeneous chemiluminescence analysis method and application thereof
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CN116754757A (en) * 2019-07-19 2023-09-15 上海索昕生物科技有限公司 Donor particle for homogeneous chemiluminescence analysis and application thereof
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CN112240937B (en) * 2019-07-19 2023-06-13 科美博阳诊断技术(上海)有限公司 Application of donor reagent in diagnosis of bacterial inflammatory diseases of subject infection
CN110441531B (en) * 2019-08-17 2022-08-23 宁波奥丞生物科技有限公司 Kit for detecting procalcitonin in blood and preparation method thereof
CN110895283A (en) * 2019-12-10 2020-03-20 宁波奥丞生物科技有限公司 High-sensitivity D-dimer detection kit and use method thereof
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CN113125714B (en) * 2019-12-31 2023-06-16 科美博阳诊断技术(上海)有限公司 Human immunodeficiency virus antibody detection kit and application thereof
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CN116400074A (en) * 2019-12-31 2023-07-07 科美博阳诊断技术(上海)有限公司 CRP detection kit and application thereof
CN113125700B (en) * 2019-12-31 2023-08-08 科美博阳诊断技术(上海)有限公司 Homogeneous detection kit for creatine kinase isozymes and application thereof
GB2616351A (en) * 2021-12-01 2023-09-06 Droplet Genomics Uab Core-shell microcapsules, manufacturing processes and uses

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
CA1190838A (en) 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
EP0136347A1 (en) 1983-02-25 1985-04-10 Covalent Technology Corporation Insoluble surfaces treated to inhibit non-specific protein binding
US4891324A (en) 1987-01-07 1990-01-02 Syntex (U.S.A.) Inc. Particle with luminescer for assays
EP0438534A4 (en) 1988-10-11 1991-09-11 Coulter Corporation Immunoreactant carriers having a novel biocompatible intermediate coating and process of making same
US5466609A (en) * 1990-10-31 1995-11-14 Coulter Corporation Biodegradable gelatin-aminodextran particle coatings of and processes for making same
US5169754A (en) * 1990-10-31 1992-12-08 Coulter Corporation Biodegradable particle coatings having a protein covalently immobilized by means of a crosslinking agent and processes for making same
US5639620A (en) * 1990-10-31 1997-06-17 Coulter Corporation Polymeric particles having a biodegradable gelatin or aminodextran coating and process for making same
AU652804B2 (en) 1991-01-19 1994-09-08 Meito Sangyo Kabushiki Kaisha Composition containing ultrafine particles of magnetic metal oxide
FR2672128B1 (en) 1991-01-28 1995-08-18 Cis Bio Int METHOD FOR MEASURING THE LUMINESCENCE EMITTED IN A LUMINESCENCE ASSAY.
US5578498A (en) 1991-05-22 1996-11-26 Behringwerke Ag Metal chelate containing compositions for use in chemiluminescent assays
IL101945A (en) 1991-05-22 1998-02-08 Behringwerke Ag Methods for determining an analyte using induced luminescence, and kits therefor
DE4117782C2 (en) * 1991-05-28 1997-07-17 Diagnostikforschung Inst Nanocrystalline magnetic iron oxide particles, processes for their production and diagnostic and / or therapeutic agents
WO1994002486A1 (en) 1992-07-20 1994-02-03 Syntex (U.S.A.) Inc. Novel chemiluminescent compounds and methods of use
WO1994003501A1 (en) * 1992-08-05 1994-02-17 Meito Sangyo Kabushiki Kaisha Small-diameter composite composed of water-soluble carboxypolysaccharide and magnetic iron oxide
DE69331970T2 (en) 1992-10-15 2002-12-19 Coulter Int Corp PARTICLES WITH GELATINE-AMINODEXTRAN COVERS AND METHOD FOR THE PRODUCTION THEREOF
EP0716746B1 (en) 1993-09-03 1999-03-17 Behringwerke Ag Fluorescent oxygen channeling immunoassays
DE4428851C2 (en) 1994-08-04 2000-05-04 Diagnostikforschung Inst Nanoparticles containing iron, their production and application in diagnostics and therapy
DE19509694A1 (en) 1995-03-08 1996-09-19 Schering Ag Use of magnetites to determine the perfusion of human tissue using MR diagnostics
US5786161A (en) 1996-06-06 1998-07-28 Miltenyi Biotec. Gmbh Isolation and characterization of allergen-binding cells for diagnosis of hypersensitivity
US6231982B1 (en) 1997-12-10 2001-05-15 Dade Behring Inc. Particle reagents having reduced matrix effects and containing an aldehyde-reactive functional group

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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JP2003526786A (en) 2003-09-09
DE60035127T2 (en) 2008-02-14
ES2287006T3 (en) 2007-12-16
DE60035127D1 (en) 2007-07-19
EP1264181A1 (en) 2002-12-11
CA2400993C (en) 2011-06-07
JP4448642B2 (en) 2010-04-14
EP1264181B1 (en) 2007-06-06
US7179660B1 (en) 2007-02-20
WO2001067105A1 (en) 2001-09-13

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